Single-Janus Rod Tracking Reveals the “Rock-and-Roll” of Endosomes in Living Cells

Gao, Yuan; Anthony, Stephen Michael; Yi, Yi; Li, Wenqian; Yu, Yanqi; Yu, Yan

doi:10.1021/acs.langmuir.7b02804

Single-Janus Rod Tracking Reveals the “Rock-and-Roll” of Endosomes in Living Cells

Journal Article · Mon Sep 25 00:00:00 EDT 2017 · Langmuir

DOI:https://doi.org/10.1021/acs.langmuir.7b02804· OSTI ID:1466758

Gao, Yuan ^[1]; Anthony, Stephen Michael ^[2]; Yi, Yi ^[1]; Li, Wenqian ^[1]; Yu, Yanqi ^[1]; ^[1]

Indiana Univ., Bloomington, IN (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Endosomes in cells are known to move directionally along microtubules, but their rotational dynamics have rarely been investigated. Even less is known, specifically, about the rotation of nonspherical endosomes. Here we report a single-Janus rod rotational tracking study to reveal the rich rotational dynamics of rod-shaped endosomes in living cells. The rotational reporters were Janus rods that display patches of different fluorescent colors on opposite sides along their long axes. When the Janus rods are wrapped tightly inside endosomes, their shape and optical anisotropy allow the simultaneous measurements of all three rotational angles (in-plane, out-of-plane, and longitudinal) and the translational motion of single endosomes with high spatiotemporal resolutions. We demonstrate that endosomes undergo in-plane rotation and rolling during intracellular transport and that such rotational dynamics are driven by rapid microtubule fluctuations. We reveal for the first time the “rock-and-roll” of endosomes in living cells and how the intracellular environment modifies such rotational dynamics. As a result, this study demonstrates a unique application of Janus particles as imaging probes in the elucidation of fundamental biological questions.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1466758

Report Number(s):: SAND--2018-8864J; 667025

Journal Information:: Langmuir, Journal Name: Langmuir Journal Issue: 3 Vol. 34; ISSN 0743-7463

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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