ER network homeostasis is critical for plant endosome streaming and endocytosis

Stefano, Giovanni; Renna, Luciana; Lai, YaShiuan; Slabaugh, Erin; Mannino, Nicole; Buono, Rafael A.; Otegui, Marisa S.; Brandizzi, Federica

doi:10.1038/celldisc.2015.33

ER network homeostasis is critical for plant endosome streaming and endocytosis

Journal Article · Tue Nov 17 00:00:00 EST 2015 · Cell Discovery

DOI:https://doi.org/10.1038/celldisc.2015.33· OSTI ID:1623746

Stefano, Giovanni ^[1]; Renna, Luciana ^[2]; Lai, YaShiuan ^[2]; Slabaugh, Erin ^[2]; Mannino, Nicole ^[2]; ^[3]; Otegui, Marisa S. ^[4]; Brandizzi, Federica ^[2]

Michigan State University, East Lansing, MI (United States). MSU-DOE Plant Research Lab and Plant Biology Department; DOE/OSTI
Michigan State University, East Lansing, MI (United States). MSU-DOE Plant Research Lab and Plant Biology Department
University of Wisconsin-Madison, Madison, WI (United States). Dept. of Botany; University of Wisconsin-Madison, Madison, WI (United States). R.M. Bock Laboratories Cell and Molecular Biology
University of Wisconsin-Madison, Madison, WI (United States). Dept. of Botany; University of Wisconsin-Madison, Madison, WI (United States). R.M. Bock Laboratories Cell and Molecular Biology; University of Wisconsin-Madison, Madison, WI (United States). Dept. of Genetics

Eukaryotic cells internalize cargo at the plasma membrane via endocytosis, a vital process that is accomplished through a complex network of endosomal organelles. In mammalian cells, the ER is in close association with endosomes and regulates their fission. Nonetheless, the physiological role of such interaction on endocytosis is yet unexplored. Here, we probed the existence of ER–endosome association in plant cells and assayed its physiological role in endocytosis. Through live-cell imaging and electron microscopy studies, we established that endosomes are extensively associated with the plant ER, supporting conservation of interaction between heterotypic organelles in evolutionarily distant kingdoms. Furthermore, by analyzing ER–endosome dynamics in genetic backgrounds with defects in ER structure and movement, we also established that the ER network integrity is necessary for homeostasis of the distribution and streaming of various endosome populations as well as for efficient endocytosis. These results support a novel model that endocytosis homeostasis depends on a spatiotemporal control of the endosome dynamics dictated by the ER membrane network.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1623746

Journal Information:: Cell Discovery, Journal Name: Cell Discovery Journal Issue: 1 Vol. 1; ISSN 2056-5968

Publisher:: Nature Research (Springer Nature)Copyright Statement

Country of Publication:: United States

Language:: English

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