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Title: Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii

Abstract

The unicellular green alga Chlamydomonas reinhardtii is a valuable experimental system in plant biology for studying metal homeostasis. Analyzing transcriptional regulation with promoter-fusion constructs in C. reinhardtii is a powerful method for connecting metal-responsive regulation with cis-regulatory elements, but overcoming expression-level variability between transformants and optimizing experimental conditions can be laborious. Here, we provide detailed protocols for the high-throughput cultivation of C. reinhardtii and assaying Venus fluorescence as a reporter for promoter activity. We also describe procedural considerations for relating metal supply to transcriptional activity.

Authors:
ORCiD logo
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1466574
Report Number(s):
BNL-208004-2018-BOOK
DOE Contract Number:  
SC0012704
Resource Type:
Book
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; algae; iron; zinc; copper; yellow fluorescent protein

Citation Formats

Blaby, Crysten. Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii. United States: N. p., 2018. Web.
Blaby, Crysten. Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii. United States.
Blaby, Crysten. Thu . "Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii". United States. https://www.osti.gov/servlets/purl/1466574.
@article{osti_1466574,
title = {Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii},
author = {Blaby, Crysten},
abstractNote = {The unicellular green alga Chlamydomonas reinhardtii is a valuable experimental system in plant biology for studying metal homeostasis. Analyzing transcriptional regulation with promoter-fusion constructs in C. reinhardtii is a powerful method for connecting metal-responsive regulation with cis-regulatory elements, but overcoming expression-level variability between transformants and optimizing experimental conditions can be laborious. Here, we provide detailed protocols for the high-throughput cultivation of C. reinhardtii and assaying Venus fluorescence as a reporter for promoter activity. We also describe procedural considerations for relating metal supply to transcriptional activity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}

Book:
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