High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner

Gao, Yuan; Xu, Weihe; Shi, Wuxian; Soares, Alexei; Jakoncic, Jean; Myers, Stuart; Martins, Bruno; Skinner, John; Liu, Qun; Bernstein, Herbert; McSweeney, Sean; Nazaretski, Evgeny; Fuchs, Martin R.

doi:10.1107/S1600577518010354

Title: High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner

Journal Article · Thu Aug 23 00:00:00 EDT 2018 · Journal of Synchrotron Radiation (Online)

DOI:https://doi.org/10.1107/S1600577518010354· OSTI ID:1466078

Gao, Yuan; Xu, Weihe; Shi, Wuxian; Soares, Alexei; Jakoncic, Jean; Myers, Stuart; Martins, Bruno; Skinner, John;

; Bernstein, Herbert; McSweeney, Sean;

;

The Frontier Microfocus Macromolecular Crystallography (FMX) beamline at the National Synchrotron Light Source II with its 1 µm beam size and photon flux of 3 × 10¹²photons s⁻¹at a photon energy of 12.66 keV has reached unprecedented dose rates for a structural biology beamline. The high dose rate presents a great advantage for serial microcrystallography in cutting measurement time from hours to minutes. To provide the instrumentation basis for such measurements at the full flux of the FMX beamline, a high-speed, high-precision goniometer based on a uniqueXYZpiezo positioner has been designed and constructed. The piezo-based goniometer is able to achieve sub-100 nm raster-scanning precision at over 10 grid-linepairs s⁻¹frequency for fly scans of a 200 µm-wide raster. The performance of the scanner in both laboratory and serial crystallography measurements up to the maximum frame rate of 750 Hz of the Eiger 16M's 4M region-of-interest mode has been verified in this work. This unprecedented experimental speed significantly reduces serial-crystallography data collection time at synchrotrons, allowing utilization of the full brightness of the emerging synchrotron radiation facilities.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Laboratory Directed Research and Development (LDRD) Program; National Institutes of Health (NIH)

Grant/Contract Number:: SC0012704; P41GM111244

OSTI ID:: 1466078

Alternate ID(s):: OSTI ID: 1476286

Report Number(s):: BNL-209117-2018-JAAM; JSYRES; PII: S1600577518010354

Journal Information:: Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Vol. 25 Journal Issue: 5; ISSN 1600-5775

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: Denmark

Language:: English

Citation Metrics:

Cited by: 16 works

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