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Office of Scientific and Technical Information
 

Structural and Molecular Characterization of the Hemagglutinin from the Fifth-Epidemic-Wave A(H7N9) Influenza Viruses

Journal Article · · Journal of Virology
DOI:https://doi.org/10.1128/JVI.00375-18· OSTI ID:1463715
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The avian influenza A(H7N9) virus continues to cause human infections in China and is a major ongoing public health concern. Five epidemic waves of A(H7N9) infection have occurred since 2013, and the recent fifth epidemic wave saw the emergence of two distinct lineages with elevated numbers of human infection cases and broader geographic distribution of viral diseases compared to the first four epidemic waves. Moreover, highly pathogenic avian influenza (HPAI) A(H7N9) viruses were also isolated during the fifth epidemic wave. Here, we present a detailed structural and biochemical analysis of the surface hemagglutinin (HA) antigen from viruses isolated during this recent epidemic wave. Results highlight that, compared to the 2013 virus HAs, the fifth-wave virus HAs remained a weak binder to human glycan receptor analogs. We also studied three mutations, V177K-K184T-G219S, that were recently reported to switch a 2013 A(H7N9) HA to human-type receptor specificity. Our results indicate that these mutations could also switch the H7 HA receptor preference to a predominantly human binding specificity for both fifth-wave H7 HAs analyzed in this study.

IMPORTANCEThe A(H7N9) viruses circulating in China are of great public health concern. Here, we report a molecular and structural study of the major surface proteins from several recent A(H7N9) influenza viruses. Our results improve the understanding of these evolving viruses and provide important information on their receptor preference that is central to ongoing pandemic risk assessment.

Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
OTHER
OSTI ID:
1463715
Journal Information:
Journal of Virology, Vol. 92, Issue 16; ISSN 0022-538X
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
ENGLISH

References (46)

Receptor binding properties of human and animal H1 influenza virus isolates November 1989
Potential for transmission of avian influenza viruses to pigs September 1994
Development of Framework for Assessing Influenza Virus Pandemic Risk August 2015
Microbatch crystallization under oil — a new technique allowing many small-volume crystallization trials August 1992
Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation January 1981
Structure and Receptor Complexes of the Hemagglutinin from a Highly Pathogenic H7N7 Influenza Virus June 2012
Effect of receptor binding domain mutations on receptor binding and transmissibility of avian influenza H5N1 viruses April 2011
Rapid adaptation of avian H7N9 virus in pigs March 2014
Coot model-building tools for molecular graphics November 2004
Use of TLS parameters to model anisotropic displacements in macromolecular refinement January 2001
Structural Analysis of the Hemagglutinin from the Recent 2013 H7N9 Influenza Virus September 2013
The 150-Loop Restricts the Host Specificity of Human H10N8 Influenza Virus April 2017
Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses April 2016
Update: Increase in Human Infections with Novel Asian Lineage Avian Influenza A(H7N9) Viruses During the Fifth Epidemic — China, October 1, 2016–August 7, 2017 September 2017
Antigenic and Genetic Characteristics of Swine-Origin 2009 A(H1N1) Influenza Viruses Circulating in Humans May 2009
Epidemiology, Evolution, and Pathogenesis of H7N9 Influenza Viruses in Five Epidemic Waves since 2013 in China September 2017
Adaptation of novel H7N9 influenza A virus to human receptors October 2013
Role of Substitutions in the Hemagglutinin in the Emergence of the 1968 Pandemic Influenza Virus September 2015
PHENIX: a comprehensive Python-based system for macromolecular structure solution January 2010
Clusters of Human Infection and Human-to-Human Transmission of Avian Influenza A(H7N9) Virus, 2013–2017 February 2018
Two Outbreak Sources of Influenza A (H7N9) Viruses Have Been Established in China March 2016
Transmission of influenza A viruses May 2015
Structure of the Uncleaved Human H1 Hemagglutinin from the Extinct 1918 Influenza Virus March 2004
Structure and Receptor Binding Preferences of Recombinant Hemagglutinins from Avian and Human H6 and H10 Influenza A Virus Subtypes February 2015
Genesis and Spread of Newly Emerged Highly Pathogenic H7N9 Avian Viruses in Mainland China September 2017
MolProbity: all-atom contacts and structure validation for proteins and nucleic acids May 2007
Biological characterisation of the emerged highly pathogenic avian influenza (HPAI) A(H7N9) viruses in humans, in mainland China, 2016 to 2017 May 2017
Receptor Specificity in Human, Avian, and Equine H2 and H3 Influenza Virus Isolates November 1994
On the origin of the human influenza virus subtypes H2N2 and H3N2 June 1978
Early Alterations of the Receptor-Binding Properties of H1, H2, and H3 Avian Influenza Virus Hemagglutinins after Their Introduction into Mammals September 2000
The origin of the 1918 pandemic influenza virus: a continuing enigma September 2003
The predicted antigenicity of the haemagglutinin of the 1918 Spanish influenza pandemic suggests an avian origin December 2001
Inefficient Transmission of H5N1 Influenza Viruses in a Ferret Contact Model April 2007
Host adaptation and transmission of influenza A viruses in mammals January 2014
Structures of Receptor Complexes of a North American H7N2 Influenza Hemagglutinin with a Loop Deletion in the Receptor Binding Site September 2010
In vitro evolution of H5N1 avian influenza virus toward human-type receptor specificity January 2012
Receptor binding by an H7N9 influenza virus from humans June 2013
A Highly Pathogenic Avian H7N9 Influenza Virus Isolated from A Human Is Lethal in Some Ferrets Infected via Respiratory Droplets November 2017
Three mutations switch H7N9 influenza to human-type receptor specificity June 2017
[20] Processing of X-ray diffraction data collected in oscillation mode January 1997
Seasonality of Influenza A(H7N9) Virus in China—Fitting Simple Epidemic Models to Human Cases March 2016
Haemagglutinin mutations responsible for the binding of H5N1 influenza A viruses to human-type receptors November 2006
Likelihood-enhanced fast translation functions March 2005
Efficient Transmission of Swine‐Adapted but Not Wholly Avian Influenza Viruses among Pigs and from Pigs to Ferrets December 2009
Human infections with recently-emerging highly pathogenic H7N9 avian influenza virus in China July 2017
Use of Influenza Risk Assessment Tool for Prepandemic Preparedness March 2018

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