Three mutations switch H7N9 influenza to human-type receptor specificity

de Vries, Robert P.; Peng, Wenjie; Grant, Oliver C.; Thompson, Andrew J.; Zhu, Xueyong; Bouwman, Kim M.; de la Pena, Alba T. Torrents; van Breemen, Marielle J.; Ambepitiya Wickramasinghe, Iresha N.; de Haan, Cornelis A. M.; Yu, Wenli; McBride, Ryan; Sanders, Rogier W.; Woods, Robert J.; Verheije, Monique H.; Wilson, Ian A.; Paulson, James C.; Fernandez-Sesma, Ana

doi:10.1371/journal.ppat.1006390

Three mutations switch H7N9 influenza to human-type receptor specificity

Journal Article · Thu Jun 15 00:00:00 EDT 2017 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1006390· OSTI ID:1400283

de Vries, Robert P. ^[1]; ^[2]; Grant, Oliver C. ^[3]; Thompson, Andrew J. ^[2]; Zhu, Xueyong ^[2]; Bouwman, Kim M. ^[4]; de la Pena, Alba T. Torrents ^[5]; van Breemen, Marielle J. ^[5]; Ambepitiya Wickramasinghe, Iresha N. ^[4]; ^[4]; Yu, Wenli ^[2]; ^[2]; Sanders, Rogier W. ^[6]; Woods, Robert J. ^[3]; ^[4]; Wilson, Ian A. ^[2]; ^[2]; Fernandez-Sesma, Ana ^[7]

The Scripps Research Inst., La Jolla, CA (United States); Utrecht Univ. (The Netherlands)
The Scripps Research Inst., La Jolla, CA (United States)
Univ. of Georgia, Athens, GA (United States)
Utrecht Univ. (The Netherlands)
Univ. of Amsterdam (The Netherlands)
Univ. of Amsterdam (The Netherlands); Weil Medical College of Cornell Univ., New York, NY (United States)
Icahn School of Medicine at Mount Sinai, New York City, NY (United States)

The avian H7N9 influenza outbreak in 2013 resulted from an unprecedented incidence of influenza transmission to humans from infected poultry. The majority of human H7N9 isolates contained a hemagglutinin (HA) mutation (Q226L) that has previously been associated with a switch in receptor specificity from avian-type (NeuAcα2-3Gal) to human-type (NeuAcα2-6Gal), as documented for the avian progenitors of the 1957 (H2N2) and 1968 (H3N2) human influenza pandemic viruses. While this raised concern that the H7N9 virus was adapting to humans, the mutation was not sufficient to switch the receptor specificity of H7N9, and has not resulted in sustained transmission in humans. To determine if the H7 HA was capable of acquiring human-type receptor specificity, we conducted mutation analyses. Remarkably, three amino acid mutations conferred a switch in specificity for human-type receptors that resembled the specificity of the 2009 human H1 pandemic virus, and promoted binding to human trachea epithelial cells.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Cancer Inst.; National Center for Research Resources; National Inst. of Health; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1400283

Journal Information:: PLoS Pathogens, Journal Name: PLoS Pathogens Journal Issue: 6 Vol. 13; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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