Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions

Youngquist, J. Tyler; Korosh, Travis C.; Pfleger, Brian F.

doi:10.1007/s10295-016-1846-3

Title: Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions

Journal Article · Mon May 01 00:00:00 EDT 2017 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-016-1846-3· OSTI ID:1924603

Youngquist, J. Tyler ^[1]; Korosh, Travis C. ^[2]; Pfleger, Brian F. ^[3]

0000 0001 2167 3675 grid.14003.36 Department of Chemical and Biological Engineering University of Wisconsin-Madison 3629 Engineering Hall, 1415 Engineering Drive Madison WI USA
0000 0001 2167 3675 grid.14003.36 Department of Chemical and Biological Engineering University of Wisconsin-Madison 3629 Engineering Hall, 1415 Engineering Drive Madison WI USA, 0000 0001 2167 3675 grid.14003.36 Graduate Program in Environmental Chemistry and Technology University of Wisconsin-Madison Madison WI USA
0000 0001 2167 3675 grid.14003.36 Department of Chemical and Biological Engineering University of Wisconsin-Madison 3629 Engineering Hall, 1415 Engineering Drive Madison WI USA, 0000 0001 2167 3675 grid.14003.36 Microbiology Doctoral Training Program, University of Wisconsin-Madison Madison WI USA

Abstract Free fatty acids (FFA) are an attractive platform chemical that serves as a functional intermediate in metabolic pathways for producing oleochemicals. Many groups have established strains of Escherichia coli capable of producing various chain-length mixtures of FFA by heterologous expression of acyl-ACP thioesterases. For example, high levels of dodecanoic acid are produced by an E. coli strain expressing the Umbellularia californica FatB2 thioesterase, BTE. Prior studies achieved high dodecanoic acid yields and productivities under phosphate-limiting media conditions. In an effort to understand the metabolic and physiological changes that led to increased FFA production, the transcriptome of this strain was assessed as a function of nutrient limitation and growth rate. FFA generation under phosphate limitation led to consistent changes in transporter expression, osmoregulation, and central metabolism. Guided by these results, targeted knockouts led to a further ~11 % in yield in FFA.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: BER DE-FC02-07ER64494; FC02-07ER64494

OSTI ID:: 1924603

Alternate ID(s):: OSTI ID: 1463132

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Vol. 44 Journal Issue: 4-5; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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Cited by: 5 works

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