Microbial Community Structure and Functional Potential Along a Hypersaline Gradient

Kimbrel, Jeffrey A.; Ballor, Nicholas; Wu, Yu-Wei; David, Maude M.; Hazen, Terry C.; Simmons, Blake A.; Singer, Steven W.; Jansson, Janet K.

doi:10.3389/fmicb.2018.01492

Title: Microbial Community Structure and Functional Potential Along a Hypersaline Gradient

Journal Article · Tue Jul 10 00:00:00 EDT 2018 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2018.01492· OSTI ID:1459672

Kimbrel, Jeffrey A.; Ballor, Nicholas; Wu, Yu-Wei; David, Maude M.; Hazen, Terry C.; Simmons, Blake A.; Singer, Steven W.; Jansson, Janet K.

Salinity is one of the strongest environmental drivers of microbial evolution and community composition. Here we aimed to determine the impact of salt concentrations (2.5, 7.5, and 33.2%) on the microbial community structure of reclaimed saltern ponds near San Francisco, California, and to discover prospective enzymes with potential biotechnological applications. Community compositions were determined by 16S rRNA amplicon sequencing revealing both higher richness and evenness in the pond sediments compared to the water columns. Co-occurrence network analysis additionally uncovered the presence of microbial seed bank communities, potentially primed to respond to rapid changes in salinity. In addition, functional annotation of shotgun metagenomic DNA showed different capabilities if the microbial communities at different salinities for methanogenesis, amino acid metabolism, and carbohydrate-active enzymes. There was an overall shift with increasing salinity in the functional potential for starch degradation, and a decrease in degradation of cellulose and other oligosaccharides. Further, many carbohydrate-active enzymes identified have acidic isoelectric points that have potential biotechnological applications, including deconstruction of biofuel feedstocks under high ionic conditions. Metagenome-assembled genomes (MAGs) of individual halotolerant and halophilic microbes were binned revealing a variety of carbohydrate-degrading potential of individual pond inhabitants.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830; AC05-00OR22725

OSTI ID:: 1459672

Alternate ID(s):: OSTI ID: 1461649; OSTI ID: 1471857

Report Number(s):: PNNL-SA-126820; 1492

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 9; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: Switzerland

Language:: English

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Cited by: 27 works

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Figures / Tables (9)

Table 1(p. 2)

(A) Richness and Evenness metrics for triplicates at each sample site (Pond A23 sediments has only a single replicate) (B) Bray-Curtis Dissimilarity dendrogram of all sample sites, color coded by sampled pond with assignment into seven groups at a cut-off of 0.6. (C)Relative abundance of the 8 most abundant phyla grouped by sample site." data-ostiid="1459672">

Table 3, Continued(p. 11)

Figure 5(p. 12)

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59 BASIC BIOLOGICAL SCIENCES
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