Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

Romero Victorica, Matias; Soria, Marcelo A.; Batista-García, Ramón Alberto; Ceja-Navarro, Javier A.; Vikram, Surendra; Ortiz, Maximiliano; Ontañon, Ornella; Ghio, Silvina; Martínez-Ávila, Liliana; Quintero García, Omar Jasiel; Etcheverry, Clara; Campos, Eleonora; Cowan, Donald; Arneodo, Joel; Talia, Paola M.

doi:10.1038/s41598-020-60850-5

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

Journal Article · Mon Mar 02 00:00:00 EST 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-60850-5· OSTI ID:1615315

^[1]; ^[2]; Batista-García, Ramón Alberto ^[3]; ^[4]; Vikram, Surendra ^[5]; Ortiz, Maximiliano ^[5]; Ontañon, Ornella ^[1]; Ghio, Silvina ^[1]; Martínez-Ávila, Liliana ^[3]; Quintero García, Omar Jasiel ^[3]; Etcheverry, Clara ^[6]; Campos, Eleonora ^[1]; Cowan, Donald ^[5]; Arneodo, Joel ^[1]; Talia, Paola M. ^[1]

Consejo Nacional de investigaciones Científicas y Tecnológicas (CONICET), Hurlingham, Buenos Aires (Argentina). Inst. de Agrobiotecnología y Biología Molecular (IABIMO), Inst. Nacional de Tecnología Agropecuaria (INTA)
Univ. de Buenos Aires, Ciudad Autónoma de Buenos Aires (Argentina). Inst. de Investigaciones en Biociencias Agrícolas y Ambientales (INBA CONICET)
Univ. Autónoma del Estado Morelos, Cuernavaca, Morelos (Mexico). Centro de Investigación en Dinámica Celular, Inst. de Investigación en Ciencias Básicas y Aplicadas
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
Univ. of Pretoria, Pretoria (South Africa)
Univ. Nacional del Nordeste, Corrientes (Argentina)

In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1615315

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 10; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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