Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein: A Force-Pulling Study

Leroueil, Pascale R.; DiMaggio, Stassi; Leistra, Abigail N.; Blanchette, Craig D.; Orme, Christine; Sinniah, Kumar; Orr, Bradford G.; Banaszak Holl, Mark M.

doi:10.1021/acs.jpcb.5b05391

Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein: A Force-Pulling Study

Journal Article · Mon Aug 10 04:00:00 EDT 2015 · Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry

DOI:https://doi.org/10.1021/acs.jpcb.5b05391· OSTI ID:1458700

Leroueil, Pascale R. ^[1]; DiMaggio, Stassi ^[2]; Leistra, Abigail N. ^[3]; Blanchette, Craig D. ^[4]; Orme, Christine ^[4]; Sinniah, Kumar ^[3]; Orr, Bradford G. ^[5]; Banaszak Holl, Mark M. ^[1]

Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Chemistry
Xavier Univ., New Orleans, LA (United States). Dept. of Chemistry
Calvin College, Grand Rapids, MI (United States). Dept. of Chemistry & Biochemistry
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physical and Life Sciences Division
Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Physics

In this paper, atomic force microscopy force-pulling experiments have been used to measure the binding forces between folic acid (FA) conjugated poly(amidoamine) (PAMAM) dendrimers and folate binding protein (FBP). The generation 5 (G5) PAMAM conjugates contained an average of 2.7, 4.7, and 7.2 FA per dendrimer. The most probable rupture force was measured to be 83, 201, and 189 pN for G5-FA_2.7, G5-FA_4.7, and G5-FA_7.2, respectively. Folic acid blocking experiments for G5-FA_7.2 reduced the frequency of successful binding events and increased the magnitude of the average rupture force to 274 pN. Finally, the force data are interpreted as arising from a network of van der Waals and electrostatic interactions that form between FBP and G5 PAMAM dendrimer, resulting in a binding strength far greater than that expected for an interaction between FA and FBP alone.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of Michigan, Ann Arbor, MI (United States)

Sponsoring Organization:: USDOE; National Inst. of Health (NIH) (United States)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1458700

Report Number(s):: LLNL-JRNL--742632; 896737

Journal Information:: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry, Journal Name: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry Journal Issue: 35 Vol. 119; ISSN 1520-6106

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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