Structure-based analysis of CysZ-mediated cellular uptake of sulfate

Assur Sanghai, Zahra; Liu, Qun; Clarke, Oliver B.; Belcher-Dufrisne, Meagan; Wiriyasermkul, Pattama; Giese, M. Hunter; Leal-Pinto, Edgar; Kloss, Brian; Tabuso, Shantelle; Love, James; Punta, Marco; Banerjee, Surajit; Rajashankar, Kanagalaghatta R.; Rost, Burkhard; Logothetis, Diomedes; Quick, Matthias; Hendrickson, Wayne A.; Mancia, Filippo

doi:10.7554/eLife.27829

Structure-based analysis of CysZ-mediated cellular uptake of sulfate

Journal Article · Thu May 24 00:00:00 EDT 2018 · eLife

DOI:https://doi.org/10.7554/eLife.27829· OSTI ID:1454809

Assur Sanghai, Zahra ^[1]; Liu, Qun ^[2]; ^[1]; Belcher-Dufrisne, Meagan ^[1]; Wiriyasermkul, Pattama ^[1]; Giese, M. Hunter ^[1]; Leal-Pinto, Edgar ^[3]; Kloss, Brian ^[4]; Tabuso, Shantelle ^[4]; Love, James ^[4]; Punta, Marco ^[5]; Banerjee, Surajit ^[6]; Rajashankar, Kanagalaghatta R. ^[6]; Rost, Burkhard ^[7]; Logothetis, Diomedes ^[3]; Quick, Matthias ^[1]; Hendrickson, Wayne A. ^[8]; ^[1]

Columbia Univ., New York, NY (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)
Virginia Commonwealth Univ. School of Medicine, Richmond, VA (United States)
New York Structural Biology Center, New York, NY (United States)
Univ. Pierre et Marie Curie, Paris (France)
Cornell Univ., NE-CAT, Argonne, IL (United States)
Technical Univ. of Munich, Munich (Germany)
Columbia Univ., New York, NY (United States); Univ. Pierre et Marie Curie, Paris (France)

Sulfur, most abundantly found in the environment as sulfate (SO₄^2-), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO₄^2- at the molecular level is limited. CysZ has been described as a SO₄^2- permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO₄^2- binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO₄^2- across membranes. CysZ structures from three different bacterial species display a hitherto unknown fold and have subunits organized with inverted transmembrane topology. CysZ from Pseudomonas denitrificans assembles as a trimer of antiparallel dimers and the CysZ structures from two other species recapitulate dimers from this assembly. In conclusion, mutational studies highlight the functional relevance of conserved CysZ residues.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0012704

OSTI ID:: 1454809

Alternate ID(s):: OSTI ID: 1440601

Report Number(s):: BNL-205770-2018-JAAM

Journal Information:: eLife, Journal Name: eLife Vol. 7; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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