Structure-based analysis of CysZ-mediated cellular uptake of sulfate

Assur Sanghai, Zahra; Liu, Qun; Clarke, Oliver B.; Belcher-Dufrisne, Meagan; Wiriyasermkul, Pattama; Giese, M. Hunter; Leal-Pinto, Edgar; Kloss, Brian; Tabuso, Shantelle; Love, James; Punta, Marco; Banerjee, Surajit; Rajashankar, Kanagalaghatta R.; Rost, Burkhard; Logothetis, Diomedes; Quick, Matthias; Hendrickson, Wayne A.; Mancia, Filippo

doi:10.7554/eLife.27829

Structure-based analysis of CysZ-mediated cellular uptake of sulfate

Journal Article · Thu May 24 04:00:00 EDT 2018 · eLife

DOI:https://doi.org/10.7554/eLife.27829· OSTI ID:1440601

Assur Sanghai, Zahra ^[1]; Liu, Qun ^[2]; ^[3]; Belcher-Dufrisne, Meagan ^[4]; Wiriyasermkul, Pattama ^[5]; Giese, M. Hunter ^[1]; Leal-Pinto, Edgar ^[6]; Kloss, Brian ^[7]; Tabuso, Shantelle ^[7]; Love, James ^[7]; Punta, Marco ^[8]; Banerjee, Surajit ^[9]; Rajashankar, Kanagalaghatta R. ^[9]; Rost, Burkhard ^[10]; Logothetis, Diomedes ^[6]; Quick, Matthias ^[11]; Hendrickson, Wayne A. ^[12]; ^[4]

Department of Physiology and Cellular Biophysics, Columbia University, New York, United States; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, United States
Biology Department, Brookhaven National Laboratory, Upton, United States
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, United States
Department of Physiology and Cellular Biophysics, Columbia University, New York, United States
Center for Molecular Recognition, Department of Psychiatry, Columbia University, New York, United States
Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, United States; Department of Pharmaceutical Sciences, School of Pharmacy, Bouvé College of Health Sciences, Northeastern University, Boston, United States
New York Structural Biology Center, New York, United States
Centre for Evolution and Cancer, The Institute of Cancer Research, London, United Kingdom
Department of Chemistry and Chemical Biology, Cornell University, NE-CAT, Argonne, United States
Department of Informatics, Technical University of Munich, Munich, Germany
Center for Molecular Recognition, Department of Psychiatry, Columbia University, New York, United States; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, United States
Department of Physiology and Cellular Biophysics, Columbia University, New York, United States; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, United States; New York Structural Biology Center, New York, United States

Sulfur, most abundantly found in the environment as sulfate (SO42-), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO42- at the molecular level is limited. CysZ has been described as a SO42- permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO42- binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO42- across membranes. CysZ structures from three different bacterial species display a hitherto unknown fold and have subunits organized with inverted transmembrane topology. CysZ from Pseudomonas denitrificans assembles as a trimer of antiparallel dimers and the CysZ structures from two other species recapitulate dimers from this assembly. Mutational studies highlight the functional relevance of conserved CysZ residues.

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: NIGMS

OSTI ID:: 1440601

Journal Information:: eLife, Journal Name: eLife Journal Issue: 2018 Vol. 7; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.

Country of Publication:: United States

Language:: ENGLISH

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