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A new approach to scaling up electrophoresis

Journal Article · · Separation Science and Technology
 [1]; ;  [2]
  1. Separations Technology, Wakefield, RI (United States)
  2. Univ. of Rhode Island, Kingston (United States)
Free Flow Electrophoresis (FFE) has been utilized for the separation of proteins and cells for many years, and has evolved into the most promising method of continuous separation. One of the major drawbacks inherent with FFE, however, is the thermal convection due to Joule heating which occurs whenever current is passed through a conducting solution. To provide efficient heat dissipation, the size of FFE units is restricted, which limits sample throughput. A new type of FFE design, which internally cools the separation unit by passing water through capillary tubes, has been developed and tested. Results of separations of dyes are presented, using a bed {1/4} inch thick which maintains efficient cooling.
Sponsoring Organization:
USDOE
OSTI ID:
145155
Report Number(s):
CONF-911049--
Journal Information:
Separation Science and Technology, Journal Name: Separation Science and Technology Journal Issue: 1-3 Vol. 28; ISSN 0149-6395; ISSN SSTEDS
Country of Publication:
United States
Language:
English

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