Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Lian, Jiazhang; HamediRad, Mohammad; Zhao, Huimin

doi:10.1002/biot.201700601

Title: Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Journal Article · Tue Feb 13 00:00:00 EST 2018 · Biotechnology Journal

DOI:https://doi.org/10.1002/biot.201700601· OSTI ID:1436426

^[1]; HamediRad, Mohammad ^[2];

^[3]

Zhejiang Univ., Hangzhou (China). College of Chemical and Biological Engineering, Key Lab. of Biomass Chemical Engineering of Ministry of Education
Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology, Dept. of Chemical and Biomolecular Engineering
Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology, Dept. of Chemical and Biomolecular Engineering; Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Chemistry, Biochemistry, and Bioengineering

Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Zhejiang Univ., Hangzhou (China)

Grant/Contract Number:: SC0018420; SC0018260

OSTI ID:: 1436426

Alternate ID(s):: OSTI ID: 1433560; OSTI ID: 1435935

Journal Information:: Biotechnology Journal, Vol. 13, Issue 9; ISSN 1860-6768

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 36 works

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Redirection of the Glycolytic Flux Enhances Isoprenoid Production in Saccharomyces cerevisiae Kwak, Suryang; Yun, Eun Ju; Lane, Stephan Biotechnology Journal, Vol. 15, Issue 2 https://doi.org/10.1002/biot.201900173	journal	September 2019
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Figure 1(p. 32)

Figure 2(p. 33)

Figure 3(p. 33)

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