Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications

Lian, Jiazhang; Mishra, Shekhar; Zhao, Huimin

doi:10.1016/j.ymben.2018.04.011

Title: Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications

Journal Article · Wed Apr 25 00:00:00 EDT 2018 · Metabolic Engineering

DOI:https://doi.org/10.1016/j.ymben.2018.04.011· OSTI ID:1436581

^[1]; Mishra, Shekhar ^[2]; Zhao, Huimin ^[3]

Zhejiang Univ., Hangzhou (China). Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering; University of Illinois at Urbana-Champaign, Urbana, IL (United States). Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology
University of Illinois at Urbana-Champaign, Urbana, IL (United States). Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology
University of Illinois at Urbana-Champaign, Urbana, IL (United States). Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology and Departments of Chemistry, Biochemistry, and Bioengineering

Metabolic engineering aims to develop efficient cell factories by rewiring cellular metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae has been extensively engineered to produce a wide variety of products at high levels from various feedstocks. In this paper, we summarize the recent development of metabolic engineering approaches to modulate yeast metabolism with representative examples. Particularly, we highlight new tools for biosynthetic pathway optimization (i.e. combinatorial transcriptional engineering and dynamic metabolic flux control) and genome engineering (i.e. clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system based genome engineering and RNA interference assisted genome evolution) to advance metabolic engineering in yeast. Lastly, we also discuss the challenges and perspectives for high throughput metabolic engineering.

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Research Organization:: Center for Advanced Bioenergy and Bioproducts (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420; SC0018260

OSTI ID:: 1436581

Journal Information:: Metabolic Engineering, Vol. 50; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 156 works

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