Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis

Uchida, Masaki; McCoy, Kimberly; Fukuto, Masafumi; Yang, Lin; Yoshimura, Hideyuki; Miettinen, Heini M.; LaFrance, Ben; Patterson, Dustin P.; Schwarz, Benjamin; Karty, Jonathan A.; Prevelige, Jr., Peter E.; Lee, Byeongdu; Douglas, Trevor

doi:10.1021/acsnano.7b06049

Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis

Journal Article · Mon Nov 13 00:00:00 EST 2017 · ACS Nano

DOI:https://doi.org/10.1021/acsnano.7b06049· OSTI ID:1435735

^[1]; McCoy, Kimberly ^[1]; Fukuto, Masafumi ^[2]; Yang, Lin ^[2]; Yoshimura, Hideyuki ^[3]; Miettinen, Heini M. ^[4]; LaFrance, Ben ^[4]; ^[5]; Schwarz, Benjamin ^[1]; Karty, Jonathan A. ^[1]; Prevelige, Jr., Peter E. ^[6]; ^[7]; ^[1]

Indiana Univ., Bloomington, IN (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)
Indiana Univ., Bloomington, IN (United States); Meiji Univ., Kawasaki (Japan)
Montana State Univ., Bozeman, MT (United States)
Univ. of Texas at Tyler, Tyler, TX (United States)
Univ. of Alabama at Birmingham, Birmingham, AL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)

The assembly of individual molecules into hierarchical structures is a promising strategy for developing three-dimensional materials with properties arising from interaction between the individual building blocks. Virus capsids are elegant examples of biomolecular nanostructures, which are themselves hierarchically assembled from a limited number of protein subunits. Here, we demonstrate the bio-inspired modular construction of materials with two levels of hierarchy: the formation of catalytically active individual virus-like particles (VLPs) through directed self-assembly of capsid subunits with enzyme encapsulation, and the assembly of these VLP building blocks into three-dimensional arrays. The structure of the assembled arrays was successfully altered from an amorphous aggregate to an ordered structure, with a face-centered cubic lattice, by modifying the exterior surface of the VLP without changing its overall morphology, to modulate interparticle interactions. The assembly behavior and resultant lattice structure was a consequence of interparticle interaction between exterior surfaces of individual particles and thus independent of the enzyme cargos encapsulated within the VLPs. These superlattice materials, composed of two populations of enzyme-packaged VLP modules, retained the coupled catalytic activity in a two-step reaction for isobutanol synthesis. As a result, this study demonstrates a significant step toward the bottom-up fabrication of functional superlattice materials using a self-assembly process across multiple length scales and exhibits properties and function that arise from the interaction between individual building blocks.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-06CH11357; SC0012704

OSTI ID:: 1435735

Alternate ID(s):: OSTI ID: 1460999

Report Number(s):: BNL--203582-2018-JAAM

Journal Information:: ACS Nano, Journal Name: ACS Nano Journal Issue: 2 Vol. 12; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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