Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis

Uchida, Masaki; McCoy, Kimberly; Fukuto, Masafumi; Yang, Lin; Yoshimura, Hideyuki; Miettinen, Heini M.; LaFrance, Ben; Patterson, Dustin P.; Schwarz, Benjamin; Karty, Jonathan A.; Prevelige, Jr., Peter E.; Lee, Byeongdu; Douglas, Trevor

doi:10.1021/acsnano.7b06049

Title: Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis

Journal Article · Mon Nov 13 00:00:00 EST 2017 · ACS Nano

DOI:https://doi.org/10.1021/acsnano.7b06049· OSTI ID:1435735

^[1]; McCoy, Kimberly ^[1]; Fukuto, Masafumi ^[2]; Yang, Lin ^[2]; Yoshimura, Hideyuki ^[3]; Miettinen, Heini M. ^[4]; LaFrance, Ben ^[4];

^[5]; Schwarz, Benjamin ^[1]; Karty, Jonathan A. ^[1]; Prevelige, Jr., Peter E. ^[6];

^[7];

^[1]

Indiana Univ., Bloomington, IN (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)
Indiana Univ., Bloomington, IN (United States); Meiji Univ., Kawasaki (Japan)
Montana State Univ., Bozeman, MT (United States)
Univ. of Texas at Tyler, Tyler, TX (United States)
Univ. of Alabama at Birmingham, Birmingham, AL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)

The assembly of individual molecules into hierarchical structures is a promising strategy for developing novel three-dimensional materials with potential for collective behavior and properties beyond those of the individual building blocks. Virus-like particles (VLPs), inspired by the elegant assembly of virus capsids, are examples of hierarchically assembled nanoparticles constituted from a limited number of protein subunits. Here we demonstrate the construction of bulk materials with two levels of hierarchy; the formation of catalytically active VLPs through directed enzyme encapsulation into VLPs, and self-assembly of these VLP building blocks into ordered three-dimensional lattices. The resulting materials, obtained by rational design of the exterior surface of the VLP to modulate interparticle interactions, adopt a face-centered cubic lattice that is independent of the enzyme cargo within the interior. We describe the design, characterization, and implications of a hierarchical structure composed of two enzyme packaged VLPs that are catalytically coupled to synthesize isobutanol.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0012704; AC02-06CH11357

OSTI ID:: 1435735

Alternate ID(s):: OSTI ID: 1460999

Report Number(s):: BNL-203582-2018-JAAM

Journal Information:: ACS Nano, Vol. 12, Issue 2; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 68 works

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coupled catalysis
enzyme encapsulation
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nanoreactor
self-assembly
superlattice
virus-like particle

Title: Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis

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