Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China

Vishnivetskaya, Tatiana A.; Hu, Haiyan; Van Nostrand, Joy D.; Wymore, Ann M.; Xu, Xiaohang; Qiu, Guangle; Feng, Xinbin; Zhou, Jizhong; Brown, Steven D.; Brandt, Craig C.; Podar, Mircea; Gu, Baohua; Elias, Dwayne A.

doi:10.1039/c7em00558j

Title: Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China

Journal Article · Mon Mar 05 00:00:00 EST 2018 · Environmental Science: Processes & Impacts

DOI:https://doi.org/10.1039/c7em00558j· OSTI ID:1435339

^[1]; Hu, Haiyan ^[2]; Van Nostrand, Joy D. ^[3]; Wymore, Ann M. ^[1]; Xu, Xiaohang ^[4]; Qiu, Guangle ^[4]; Feng, Xinbin ^[4]; Zhou, Jizhong ^[3];

^[1];

^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Chinese Academy of Science, Guiyang (China)
Univ. of Oklahoma, Norman, OK (United States)
Chinese Academy of Science, Guiyang (China)

Paddy soils from mercury (Hg)-contaminated rice fields in Guizhou, China were studied with respect to total mercury (THg) and methylmercury (MeHg) concentrations as well as Bacterial and Archaeal community composition. Total Hg (0.25-990 μg g^-1) and MeHg (1.3-30.5 ng g^-1) varied between samples. Pyrosequencing (454 FLX) of the hypervariable v1-v3 regions of the 16S rRNA genes showed that Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, Euryarchaeota, and Crenarchaeota were dominant in all samples. The Bacterial α-diversity was higher in samples with relatively Low THg and MeHg and decreased with increasing THg and MeHg concentrations. In contrast, Archaeal α-diversity increased with increasing of MeHg concentrations but did not correlate with changes in THg concentrations. Overall, the methylation gene hgcAB copy number increased with both increasing THg and MeHg concentrations. The microbial communities at High THg and High MeHg appear to be adapted by species that are both Hg resistant and carry hgcAB genes for MeHg production. The relatively high abundance of both sulfate-reducing δ-Proteobacteria and methanogenic Archaea, as well as their positive correlations with increasing THg and MeHg concentrations, suggests that these microorganisms are the primary Hg-methylators in the rice paddy soils in Guizhou, China.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231

OSTI ID:: 1435339

Alternate ID(s):: OSTI ID: 1434080; OSTI ID: 1479398

Journal Information:: Environmental Science: Processes & Impacts, Vol. 20, Issue 4; ISSN 2050-7887

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 33 works

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59 BASIC BIOLOGICAL SCIENCES
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Hg-methylation
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rice paddy soil
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Title: Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China

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