Long-term nitrogen fertilization decreases bacterial diversity and favors the growth of Actinobacteria and Proteobacteria in agro-ecosystems across the globe

Dai, Zhongmin; Su, Weiqin; Chen, Huaihai; Barberán, Albert; Zhao, Haochun; Yu, Mengjie; Yu, Lu; Brookes, Philip C.; Schadt, Christopher W.; Chang, Scott X.; Xu, Jianming

doi:10.1111/gcb.14163

Long-term nitrogen fertilization decreases bacterial diversity and favors the growth of Actinobacteria and Proteobacteria in agro-ecosystems across the globe

Journal Article · Thu Apr 12 00:00:00 EDT 2018 · Global Change Biology

DOI:https://doi.org/10.1111/gcb.14163· OSTI ID:1435246

Dai, Zhongmin ^[1]; Su, Weiqin ^[1]; Chen, Huaihai ^[2]; Barberán, Albert ^[3]; Zhao, Haochun ^[1]; Yu, Mengjie ^[1]; Yu, Lu ^[1]; Brookes, Philip C. ^[1]; ^[2]; Chang, Scott X. ^[4]; ^[1]

Zhejiang Univ., Hangzhou (China). Zhejiang Provincial Key Lab. of Agricultural Resources and Environment, Inst. of Soil and Water Resources and Environmental Science
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Arizona, Tucson, AZ (United States)
Univ. of Alberta, Edmonton, AB (Canada)

Long-term Elevated nitrogen (N) input from anthropogenic sources may cause soil acidification and decrease crop yield, yet the response of the belowground microbial community to long-term N input and the input of N combined with phosphorus (P) and potassium (K) is still poorly understood. Here, we explored the effect of long-term N and NPK fertilization on soil bacterial diversity and community composition using meta-analysis of a global dataset. Nitrogen fertilization decreased soil pH, and increased soil organic carbon (C) and available N contents. Bacterial taxonomic diversity was decreased by N fertilization alone, but was increased by NPK fertilization. The effect of N fertilization on bacterial diversity depends on soil texture and water management, but independent of crop type or N application rate. Both soil pH and organic C content were positively related to changes in bacterial diversity under N fertilization, while soil organic C was the dominant factor determining changes in bacterial diversity under NPK fertilization. Microbial biomass C decreased with decreasing bacterial diversity under long-term N fertilization. Nitrogen fertilization increased the relative abundance of copiotrophic bacteria (i.e. Proteobacteria and Actinobacteria), but reduced the abundance of oligotrophic taxa (i.e. Acidobacteria), consistent with the general life history strategy theory for bacteria. The relative abundance of Proteobacteria was also increased by NPK fertilization. The positive correlation between N application rate and the relative abundance of Actinobacteria indicates that increased N availability favored the growth of Actinobacteria. This first global analysis of long-term N and NPK fertilization effect on bacterial diversity and community composition suggests that N input decreases bacterial diversity but favors the growth of copiotrophic bacteria, providing a reference for nutrient management strategies for maintaining belowground microbial diversity in agro-ecosystems worldwide.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Basic Research Program of China; National Natural Science Foundation of China (NNSFC); USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1435246

Journal Information:: Global Change Biology, Journal Name: Global Change Biology Journal Issue: 8 Vol. 24; ISSN 1354-1013

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Actinobacteria
N fertilization
agro-ecosystems
bacterial diversity
community composition
microbial biomass

Long-term nitrogen fertilization decreases bacterial diversity and favors the growth of Actinobacteria and Proteobacteria in agro-ecosystems across the globe

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