Significance of Lignin S/G Ratio in Biomass Recalcitrance of Populus trichocarpa Variants for Bioethanol Production

Yoo, Chang Geun; Dumitrache, Alexandru; Muchero, Wellington; Natzke, Jace; Akinosho, Hannah; Li, Mi; Sykes, Robert W.; Brown, Steven D.; Davison, Brian; Tuskan, Gerald A.; Pu, Yunqiao; Ragauskas, Arthur J.

doi:10.1021/acssuschemeng.7b03586

Title: Significance of Lignin S/G Ratio in Biomass Recalcitrance of Populus trichocarpa Variants for Bioethanol Production

Journal Article · Wed Dec 27 00:00:00 EST 2017 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.7b03586· OSTI ID:1431420

Yoo, Chang Geun ^[1]; Dumitrache, Alexandru ^[2]; Muchero, Wellington ^[2]; Natzke, Jace ^[2]; Akinosho, Hannah ^[3]; Li, Mi ^[1]; Sykes, Robert W. ^[4]; Brown, Steven D. ^[2]; Davison, Brian ^[2]; Tuskan, Gerald A. ^[2]; Pu, Yunqiao ^[1];

^[5]

BioEnergy Science Center &, Center for BioEnergy Innovation, Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, Tennessee 37831, United States; UT−ORNL Joint Institute for Biological Science, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, Tennessee 37831, United States
BioEnergy Science Center &, Center for BioEnergy Innovation, Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, Tennessee 37831, United States
School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, United States
National Renewable Energy Laboratory, U.S. Department of Energy, 15013 Denver West Parkway, Golden, Colorado 80401, United States
BioEnergy Science Center &, Center for BioEnergy Innovation, Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, Tennessee 37831, United States; UT−ORNL Joint Institute for Biological Science, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, Tennessee 37831, United States; Department of Chemical and Biomolecular Engineering &, Center for Renewable Carbon, Department of Forestry, Wildlife, and Fisheries, University of Tennessee, 1512 Middle Drive, Knoxville, Tennessee 37996, United States

Lignin S/G ratio has been investigated as an important factor in biomass recalcitrance to bioethanol production. Because of the complexity and variety of biomass, recalcitrance was also reportedly influenced by several other factors, such as total lignin content, degree of cellulose polymerization, etc. In addition, the effect of S/G ratio on biomass conversion is not uniform across plant species. Herein, 11 Populus trichocarpa natural variants grown under the same conditions with similar total lignin content were selected to minimize the effects of other factors. The lignin S/G ratio of the selected P. trichocarpa natural variants showed negative correlations with p-hydroxybenzoate (PB) and ..beta..-5 linkage contents, while it had positive ones with ..beta..-O-4 linkage, lignin molecular weight, and ethanol production. This study showed the importance of lignin S/G ratio as an independent recalcitrance factor that may aid future energy crop engineering and biomass conversion strategies.

Cite

Export

Save

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

DOE Contract Number:: AC36-08GO28308

OSTI ID:: 1431420

Report Number(s):: NREL/JA-5100-71261

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 2; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

References (36)

The DFRC Method for Lignin Analysis. 6. A Simple Modification for Identifying Natural Acetates on Lignins Ralph, John; Lu, Fachuang Journal of Agricultural and Food Chemistry, Vol. 46, Issue 11, p. 4616-4619 https://doi.org/10.1021/jf980680d	journal	November 1998
The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray) Tuskan, G. A.; DiFazio, S.; Jansson, S. Science, Vol. 313, Issue 5793, p. 1596-1604 https://doi.org/10.1126/science.1128691	journal	September 2006
Lignin monomer composition affects Arabidopsis cell-wall degradability after liquid hot water pretreatment Li, Xu; Ximenes, Eduardo; Kim, Youngmi Biotechnology for Biofuels, Vol. 3, Issue 1 https://doi.org/10.1186/1754-6834-3-27	journal	January 2010
Lignin Structural Variation in Hardwood Species Santos, Ricardo B.; Capanema, Ewellyn A.; Balakshin, Mikhail Yu. Journal of Agricultural and Food Chemistry, Vol. 60, Issue 19 https://doi.org/10.1021/jf301276a	journal	May 2012
The effect of delignification of forest biomass on enzymatic hydrolysis Yu, Zhiying; Jameel, Hasan; Chang, Hou-min Bioresource Technology, Vol. 102, Issue 19 https://doi.org/10.1016/j.biortech.2011.07.001	journal	October 2011
Lignin content in natural Populus variants affects sugar release Studer, M. H.; DeMartini, J. D.; Davis, M. F. Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305 https://doi.org/10.1073/pnas.1009252108	journal	March 2011
Influence of Syringyl to Guaiacyl Ratio on the Structure of Natural and Synthetic Lignins Kishimoto, Takao; Chiba, Wakako; Saito, Kaori Journal of Agricultural and Food Chemistry, Vol. 58, Issue 2 https://doi.org/10.1021/jf9035172	journal	January 2010
Assessment of Lignocellulosic Biomass Using Analytical Spectroscopy: an Evolution to High-Throughput Techniques Lupoi, Jason S.; Singh, Seema; Simmons, Blake A. BioEnergy Research, Vol. 7, Issue 1 https://doi.org/10.1007/s12155-013-9352-1	journal	July 2013
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808 https://doi.org/10.1073/pnas.1100310108	journal	February 2011
Lignin modification improves fermentable sugar yields for biofuel production Chen, Fang; Dixon, Richard A. Nature Biotechnology, Vol. 25, Issue 7, p. 759-761 https://doi.org/10.1038/nbt1316	journal	June 2007
Variation in Cell Wall Composition among Forage Maize ( Zea mays L.) Inbred Lines and Its Impact on Digestibility: Analysis of Neutral Detergent Fiber Composition by Pyrolysis-Gas Chromatography-Mass Spectrometry Fontaine, Anne-Sophie; Bout, Siobhán; Barrière, Yves Journal of Agricultural and Food Chemistry, Vol. 51, Issue 27 https://doi.org/10.1021/jf034321g	journal	December 2003
Quantification of Wheat Straw Lignin Structure by Comprehensive NMR Analysis Zeng, Jijiao; Helms, Gregory L.; Gao, Xin Journal of Agricultural and Food Chemistry, Vol. 61, Issue 46 https://doi.org/10.1021/jf4030486	journal	November 2013
Significant Increases in Pulping Efficiency in C4H-F5H-Transformed Poplars: Improved Chemical Savings and Reduced Environmental Toxins Huntley, Shannon K.; Ellis, Dave; Gilbert, Margarita Journal of Agricultural and Food Chemistry, Vol. 51, Issue 21 https://doi.org/10.1021/jf034320o	journal	October 2003
Antisense Down-Regulation of 4CL Expression Alters Lignification, Tree Growth, and Saccharification Potential of Field-Grown Poplar Voelker, Steven L.; Lachenbruch, Barbara; Meinzer, Frederick C. Plant Physiology, Vol. 154, Issue 2 https://doi.org/10.1104/pp.110.159269	journal	August 2010
Erythro/threo ratio of β-O-4-5 structures as an important structural characteristic of lignin. Part 4: Variation in the erythro/threo ratio in softwood and hardwood lignins and its relation to syringyl/guaiacyl ratio Akiyama, Takuya; Goto, Hitoshi; Nawawi, Deded S. Holzforschung, Vol. 59, Issue 3 https://doi.org/10.1515/HF.2005.045	journal	May 2005
p-coumaroylated syringyl units in maize lignin: Implications for β-ether cleavage by thioacidolysis Grabber, John H.; Quideau, Stephane; Ralph, John Phytochemistry, Vol. 43, Issue 6, p. 1189-1194 https://doi.org/10.1016/S0031-9422(96)00431-1	journal	December 1996
Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose Zhao, Xuebing; Zhang, Lihua; Liu, Dehua Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 4 https://doi.org/10.1002/bbb.1331	journal	March 2012
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production Himmel, M. E.; Ding, S.-Y.; Johnson, D. K. Science, Vol. 315, Issue 5813, p. 804-807 https://doi.org/10.1126/science.1137016	journal	February 2007
Investigation of the fate of poplar lignin during autohydrolysis pretreatment to understand the biomass recalcitrance Samuel, Reichel; Cao, Shilin; Das, Birinchi K. RSC Advances, Vol. 3, Issue 16 https://doi.org/10.1039/c3ra40578h	journal	January 2013
Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels Zeng, Yining; Zhao, Shuai; Yang, Shihui Current Opinion in Biotechnology, Vol. 27 https://doi.org/10.1016/j.copbio.2013.09.008	journal	June 2014
High-resolution genetic mapping of allelic variants associated with cell wall chemistry in Populus Muchero, Wellington; Guo, Jianjun; DiFazio, Stephen P. BMC Genomics, Vol. 16, Issue 1 https://doi.org/10.1186/s12864-015-1215-z	journal	January 2015
Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase Van Acker, R.; Leple, J. -C.; Aerts, D. Proceedings of the National Academy of Sciences, Vol. 111, Issue 2 https://doi.org/10.1073/pnas.1321673111	journal	December 2013
Characterization of the Interunit Bonds of Lignin Oligomers Released by Acid-Catalyzed Selective Solvolysis of Cryptomeria japonica and Eucalyptus globulus Woods via Thioacidolysis and 2D-NMR Saito, Kaori; Kaiho, Atsushi; Sakai, Ryo Journal of Agricultural and Food Chemistry, Vol. 64, Issue 48 https://doi.org/10.1021/acs.jafc.6b04263	journal	November 2016
Detection and Determination of p-Coumaroylated Units in Lignins Lu, Fachuang; Ralph, John Journal of Agricultural and Food Chemistry, Vol. 47, Issue 5, p. 1988-1992 https://doi.org/10.1021/jf981140j	journal	May 1999
Investigating plant cell wall components that affect biomass recalcitrance in poplar and switchgrass DeMartini, Jaclyn D.; Pattathil, Sivakumar; Miller, Jeffrey S. Energy & Environmental Science, Vol. 6, Issue 3 https://doi.org/10.1039/c3ee23801f	journal	January 2013
Profiling of Oligolignols Reveals Monolignol Coupling Conditions in Lignifying Poplar Xylem Morreel, Kris; Ralph, John; Kim, Hoon Plant Physiology, Vol. 136, Issue 3 https://doi.org/10.1104/pp.104.049304	journal	October 2004
Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure Dumitrache, Alexandru; Akinosho, Hannah; Rodriguez, Miguel Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0445-x	journal	February 2016
Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin Lupoi, Jason S.; Singh, Seema; Parthasarathi, Ramakrishnan Renewable and Sustainable Energy Reviews, Vol. 49 https://doi.org/10.1016/j.rser.2015.04.091	journal	September 2015
Role of Functional Groups in Lignin Inhibition of Enzymatic Hydrolysis of Cellulose to Glucose Pan, Xuejun Journal of Biobased Materials and Bioenergy, Vol. 2, Issue 1 https://doi.org/10.1166/jbmb.2008.005	journal	March 2008
Effects on Lignin Structure of Coumarate 3-Hydroxylase Downregulation in Poplar Ralph, John; Akiyama, Takuya; Coleman, Heather D. BioEnergy Research, Vol. 5, Issue 4 https://doi.org/10.1007/s12155-012-9218-y	journal	May 2012
Preparation and relevance of a cross-coupling product between sinapyl alcohol and sinapyl p-hydroxybenzoate Lu, Fachuang; Ralph, John; Morreel, Kris Organic & Biomolecular Chemistry, Vol. 2, Issue 20 https://doi.org/10.1039/b411428k	journal	January 2004
Transgenic switchgrass ( Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: a 2-year comparative analysis of field-grown lines modified for target gene or genetic element expression Dumitrache, Alexandru; Natzke, Jace; Rodriguez, Miguel Plant Biotechnology Journal, Vol. 15, Issue 6 https://doi.org/10.1111/pbi.12666	journal	February 2017
Variation of S/G Ratio and Lignin Content in a <i>Populus</i> Family Influences the Release of Xylose by Dilute Acid Hydrolysis Davison, Brian H.; Drescher, Sadie R.; Tuskan, Gerald A. Applied Biochemistry and Biotechnology, Vol. 130, Issue 1-3 https://doi.org/10.1385/ABAB:130:1:427	journal	January 2006
Modifications in Lignin and Accumulation of Phenolic Glucosides in Poplar Xylem upon Down-regulation of Caffeoyl-Coenzyme A O-Methyltransferase, an Enzyme Involved in Lignin Biosynthesis Meyermans, Hugo; Morreel, Kris; Lapierre, Catherine Journal of Biological Chemistry, Vol. 275, Issue 47, p. 36899-36909 https://doi.org/10.1074/jbc.M006915200	journal	August 2000
Lignins: Natural polymers from oxidative coupling of 4-hydroxyphenyl- propanoids Ralph, John; Lundquist, Knut; Brunow, Gösta Phytochemistry Reviews, Vol. 3, Issue 1-2, p. 29-60 https://doi.org/10.1023/B:PHYT.0000047809.65444.a4	journal	January 2004
High-Throughput Screening of Plant Cell-Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy Sykes, Robert; Yung, Matthew; Novaes, Evandro Biofuels https://doi.org/10.1007/978-1-60761-214-8_12	book	January 2009

Cited By (7)

Fractionation of non-timber wood from Atlantic mixed forest into high-value lignocellulosic materials Sillero, Leyre; Barriga, Sebastián; Izaguirre, Nagore Taylor & Francis https://doi.org/10.6084/m9.figshare.11961039	text	January 2020
Fractionation of non-timber wood from Atlantic mixed forest into high-value lignocellulosic materials Sillero, Leyre; Barriga, Sebastián; Izaguirre, Nagore Journal of Wood Chemistry and Technology, Vol. 40, Issue 3 https://doi.org/10.1080/02773813.2020.1737132	journal	March 2020
Lignocellulosic Biomass: Understanding Recalcitrance and Predicting Hydrolysis Zoghlami, Aya; Paës, Gabriel Frontiers in Chemistry, Vol. 7 https://doi.org/10.3389/fchem.2019.00874	journal	December 2019
Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar Straub, Christopher T.; Bing, Ryan G.; Wang, Jack P. Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-01675-2	journal	March 2020
Cell wall compositional and vascular traits of hybrid poplar wood in micropropagated plants and plants propagated from root cuttings Ďurkovič, Jaroslav; Kačík, František; Husárová, Hana New Forests, Vol. 51, Issue 1 https://doi.org/10.1007/s11056-019-09723-y	journal	May 2019
Fractionation of non-timber wood from Atlantic mixed forest into high-value lignocellulosic materials Sillero, Leyre; Barriga, Sebastián; Izaguirre, Nagore Taylor & Francis https://doi.org/10.6084/m9.figshare.11961039.v1	text	January 2020
Lignin Engineering in Forest Trees Chanoca, Alexandra; de Vries, Lisanne; Boerjan, Wout Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.00912	journal	July 2019

Similar Records

Significance of Lignin S/G Ratio in Biomass Recalcitrance of Populus trichocarpa Variants for Bioethanol Production

Journal Article · Mon Dec 11 00:00:00 EST 2017 · ACS Sustainable Chemistry & Engineering · OSTI ID:1431420

Yoo, Chang Geun; Dumitrache, Alexandru; Muchero, Wellington; +9 more

Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Journal Article · Mon Oct 09 00:00:00 EDT 2017 · Green Chemistry · OSTI ID:1431420

Yoo, Chang Geun; Yang, Yongil; Pu, Yunqiao; +16 more