Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Yoo, Chang Geun; Yang, Yongil; Pu, Yunqiao; Meng, Xianzhi; Muchero, Wellington; Yee, Kelsey L.; Thompson, Olivia A.; Rodriguez, Jr., Miguel; Bali, Garima; Engle, Nancy L.; Lindquist, Erika; Singan, Vasanth; Schmutz, Jeremy; DiFazio, Stephen P.; Tschaplinski, Timothy J.; Tuskan, Gerald A.; Chen, Jin -Gui; Davison, Brian; Ragauskas, Arthur J.

doi:10.1039/C7GC02219K

Title: Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Journal Article · Mon Oct 09 00:00:00 EDT 2017 · Green Chemistry

DOI:https://doi.org/10.1039/C7GC02219K· OSTI ID:1410915

^[1];

^[1]; Meng, Xianzhi ^[2];

^[1]; Yee, Kelsey L. ^[1]; Thompson, Olivia A. ^[1];

^[1]; Bali, Garima ^[2];

^[1];

^[3];

^[3]; Schmutz, Jeremy ^[4]; DiFazio, Stephen P. ^[5];

^[1];

^[6]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Georgia Inst. of Technology, Atlanta, GA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States)
West Virginia Univ., Morgantown, WV (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Herein, the recalcitrance of natural Populus variants was elucidated in three aspects: sugar release, physicochemical properties, and relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. As a result, the trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

OSTI ID:: 1410915

Journal Information:: Green Chemistry, Vol. 19, Issue 22; ISSN 1463-9262

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

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