Effects of stress waves on cells
- LLNL
Laser induced stress waves are being used in a variety of medical applications, including drug delivery and targeted tissue disruption. Stress waves can also be an undesirable side effect in laser procedures such as ophthalmology and angioplasty. Thus, a study of the effects of stress waves on a cellular level is useful. Thermoelastic stress waves were produced using a Q-switched frequency-doubled Nd:YAG laser (@.=532nm) with a pulse duration of 4 ns. The laser radiation was delivered to an absorbing media. A thermoelastic stress wave was produced in the absorbing media and propagated into plated cells. The energy per pulse delivered to a sample and the spot size were varied. Stress waves were quantified. We assayed for cell viability and damage using two methods. The laser parameters within which cells maintain viability were investigated and thresholds for cell damage were defined. A comparison of cell damage thresholds for different cell lines was made.
- Research Organization:
- Lawrence Livermore National Lab., CA (US)
- Sponsoring Organization:
- USDOE Office of Defense Programs (DP) (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 14309
- Report Number(s):
- UCRL-JC-130131; YN0100000; YN0100000
- Country of Publication:
- United States
- Language:
- English
Similar Records
Stress wave focusing transducers
Opto-acoustic cell permeation
Biological effects of laser-induced stress waves
Conference
·
Fri May 15 00:00:00 EDT 1998
·
OSTI ID:302214
Opto-acoustic cell permeation
Conference
·
Wed Mar 08 23:00:00 EST 2000
·
OSTI ID:756840
Biological effects of laser-induced stress waves
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:240871