Effects of stress waves on cells
Laser induced stress waves are being used in a variety of medical applications, including drug delivery and targeted tissue disruption. Stress waves can also be an undesirable side effect in laser procedures such as ophthalmology and angioplasty. Thus, a study of the effects of stress waves on a cellular level is useful. Thermoelastic stress waves were produced using a Q-switched frequency-doubled Nd:YAG laser (@.=532nm) with a pulse duration of 4 ns. The laser radiation was delivered to an absorbing media. A thermoelastic stress wave was produced in the absorbing media and propagated into plated cells. The energy per pulse delivered to a sample and the spot size were varied. Stress waves were quantified. We assayed for cell viability and damage using two methods. The laser parameters within which cells maintain viability were investigated and thresholds for cell damage were defined. A comparison of cell damage thresholds for different cell lines was made.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE Office of Defense Programs (DP) (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 14309
- Report Number(s):
- UCRL-JC-130131; YN0100000; YN0100000; TRN: US0110996
- Resource Relation:
- Journal Volume: 3254; Conference: Society of Photo-Optical Instrumentation Engineers, Laser Tissue Interaction IX, San Jose, CA (US), 01/24/1998--01/30/1998; Other Information: PBD: 2 Mar 1998
- Country of Publication:
- United States
- Language:
- English
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