Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation

Lima, Cleilton S.; Rabelo, Sarita C.; Ciesielski, Peter N.; Roberto, Ines C.; Rocha, George J. M.; Driemeier, Carlos

doi:10.1021/acssuschemeng.7b04158

Title: Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation

Journal Article · Wed Jan 31 00:00:00 EST 2018 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.7b04158· OSTI ID:1430818

Lima, Cleilton S. ^[1]; Rabelo, Sarita C. ^[2]; Ciesielski, Peter N. ^[3]; Roberto, Ines C. ^[4]; Rocha, George J. M. ^[4]; Driemeier, Carlos ^[2]

Brazilian Center for Research in Energy and Materials (CNPEM), Sao Paulo (Brazil); Univ.of Sao Paulo (Brazil)
Brazilian Center for Research in Energy and Materials (CNPEM), Sao Paulo (Brazil)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ.of Sao Paulo (Brazil)

Alkaline deacetylation has emerged as a promising chemistry for pretreatments performed prior to enzymatic saccharification of lignocellulosic biomass. This process avoids complex pressurized reactors and opens new opportunities for lignin covalorization. In this work, we evaluate the chemical and morphological response of sugar cane bagasse and straw submitted to alkaline treatments. Alkaline solutions for deacetylation (0.4% w/w NaOH, 70 degrees C, 3 h) as well as proximal conditions (0.1-0.7% NaOH, 55-85 degrees C, 1-5 h) chosen by 23 experimental design were evaluated. The deacetylation treatment removes ~90% of the acetyl groups and 20-30% of the lignin from both bagasse and straw, while removal of ~20% of the xylan and glucan is observed in straw, but not in bagasse. Considering nanoscale structural alterations, neither cellulose cocrystallization (evaluated by X-ray diffraction) nor formation of lignin aggregates (evaluated by thermoporometric signature) are observed after the alkaline conditions, in contrast to observations after hydrothermal treatments. Furthermore, calorimetric thermoporometry as well as scanning and transmission electron microscopies show substantial introduction of nanoscale porosity and loosening of the tissue and cell wall structures, indicating desirable mechanical weakening and gains in enzyme accessibility. These results provide fundamental and practical knowledge for biorefineries based on alkaline deacetylation of sugar cane bagasse and straw.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1430818

Report Number(s):: NREL/JA-2700-71218

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 3; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 16 works

Citation information provided by
Web of Science

References (51)

Exploration of the ranges of the global potential of biomass for energy Hoogwijk, Monique; Faaij, André; van den Broek, Richard Biomass and Bioenergy, Vol. 25, Issue 2 https://doi.org/10.1016/S0961-9534(02)00191-5	journal	August 2003
Global biomass fuel resources Parikka, Matti Biomass and Bioenergy, Vol. 27, Issue 6 https://doi.org/10.1016/j.biombioe.2003.07.005	journal	December 2004
Sugarcane processing for ethanol and sugar in Brazil Dias, Marina Oliveira de Souza; Maciel Filho, Rubens; Mantelatto, Paulo Eduardo Environmental Development, Vol. 15 https://doi.org/10.1016/j.envdev.2015.03.004	journal	July 2015
Bioethanol from lignocelluloses: Status and perspectives in Brazil Soccol, Carlos Ricardo; Vandenberghe, Luciana Porto de Souza; Medeiros, Adriane Bianchi Pedroni Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2009.11.067	journal	July 2010
Techno-economic analysis and climate change impacts of sugarcane biorefineries considering different time horizons Junqueira, Tassia L.; Chagas, Mateus F.; Gouveia, Vera L. R. Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0722-3	journal	March 2017
Comprehensive assessment of sugarcane straw: implications for biomass and bioenergy production Menandro, Lauren Maine Santos; Cantarella, Heitor; Franco, Henrique Coutinho Junqueira Biofuels, Bioproducts and Biorefining, Vol. 11, Issue 3 https://doi.org/10.1002/bbb.1760	journal	February 2017
Features of promising technologies for pretreatment of lignocellulosic biomass Mosier, Nathan; Wyman, Charles; Dale, Bruce Bioresource Technology, Vol. 96, Issue 6, p. 673-686 https://doi.org/10.1016/j.biortech.2004.06.025	journal	April 2005
Cellulosic ethanol: status and innovation Lynd, Lee R.; Liang, Xiaoyu; Biddy, Mary J. Current Opinion in Biotechnology, Vol. 45 https://doi.org/10.1016/j.copbio.2017.03.008	journal	June 2017
Common processes drive the thermochemical pretreatment of lignocellulosic biomass Langan, Paul; Petridis, Loukas; O'Neill, Hugh M. Green Chem., Vol. 16, Issue 1 https://doi.org/10.1039/C3GC41962B	journal	January 2014
Hydrothermal processing, as an alternative for upgrading agriculture residues and marine biomass according to the biorefinery concept: A review Ruiz, Héctor A.; Rodríguez-Jasso, Rosa M.; Fernandes, Bruno D. Renewable and Sustainable Energy Reviews, Vol. 21 https://doi.org/10.1016/j.rser.2012.11.069	journal	May 2013
Pilot scale steam explosion and diluted sulfuric acid pretreatments: Comparative study aiming the sugarcane bagasse saccharification Rocha, G. J. M.; Gonçalves, A. R.; Nakanishi, S. C. Industrial Crops and Products, Vol. 74 https://doi.org/10.1016/j.indcrop.2015.05.074	journal	November 2015
Alternatives for detoxification of diluted-acid lignocellulosic hydrolyzates for use in fermentative processes: a review Mussatto, S. Bioresource Technology, Vol. 93, Issue 1 https://doi.org/10.1016/j.biortech.2003.10.005	journal	May 2004
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects Jönsson, Leif J.; Martín, Carlos Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.10.009	journal	January 2016
The fate of lignin during hydrothermal pretreatment Trajano, Heather L.; Engle, Nancy L.; Foston, Marcus Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-110	journal	January 2013
Biomass pretreatments capable of enabling lignin valorization in a biorefinery process Narron, Robert H.; Kim, Hoyong; Chang, Hou-min Current Opinion in Biotechnology, Vol. 38 https://doi.org/10.1016/j.copbio.2015.12.018	journal	April 2016
Cellulose co-crystallization and related phenomena occurring in hydrothermal treatment of sugarcane bagasse Driemeier, Carlos; Mendes, Fernanda M.; Santucci, Beatriz S. Cellulose, Vol. 22, Issue 4 https://doi.org/10.1007/s10570-015-0638-7	journal	April 2015
Cellulose Aggregation under Hydrothermal Pretreatment Conditions Silveira, Rodrigo L.; Stoyanov, Stanislav R.; Kovalenko, Andriy Biomacromolecules, Vol. 17, Issue 8 https://doi.org/10.1021/acs.biomac.6b00603	journal	July 2016
Ultrastructure of steam-exploded wood Donaldson, L. A.; Wong, K. K. Y.; Mackie, K. L. Wood Science and Technology, Vol. 22, Issue 2 https://doi.org/10.1007/BF00355846	journal	January 1988
Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment Donohoe, Bryon S.; Decker, Stephen R.; Tucker, Melvin P. Biotechnology and Bioengineering, Vol. 101, Issue 5 https://doi.org/10.1002/bit.21959	journal	December 2008
Lignin contributions to the nanoscale porosity of raw and treated lignocelluloses as observed by calorimetric thermoporometry Driemeier, Carlos; Oliveira, Marcelo M.; Curvelo, Antônio A. S. Industrial Crops and Products, Vol. 82 https://doi.org/10.1016/j.indcrop.2015.11.084	journal	April 2016
Biomass feeding for thermochemical reactors Dai, Jianjun; Cui, Heping; Grace, John R. Progress in Energy and Combustion Science, Vol. 38, Issue 5 https://doi.org/10.1016/j.pecs.2012.04.002	journal	October 2012
A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass Kim, Jun Seok; Lee, Y. Y.; Kim, Tae Hyun Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.08.085	journal	January 2016
The impacts of deacetylation prior to dilute acid pretreatment on the bioethanol process Chen, Xiaowen; Shekiro, Joseph; Franden, Mary Ann Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-8	journal	January 2012
A highly efficient dilute alkali deacetylation and mechanical (disc) refining process for the conversion of renewable biomass to lower cost sugars Chen, Xiaowen; Shekiro, Joseph; Pschorn, Thomas Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-98	journal	January 2014
DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L ⁻¹ ) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration Chen, Xiaowen; Kuhn, Erik; Jennings, Edward W. Energy & Environmental Science, Vol. 9, Issue 4 https://doi.org/10.1039/C5EE03718B	journal	January 2016
Improving Sugar Yields and Reducing Enzyme Loadings in the Deacetylation and Mechanical Refining (DMR) Process through Multistage Disk and Szego Refining and Corresponding Techno-Economic Analysis Chen, Xiaowen; Wang, Wei; Ciesielski, Peter ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 1 https://doi.org/10.1021/acssuschemeng.5b01242	journal	December 2015
Evaluation of Brazilian Sugarcane Bagasse Characterization: An Interlaboratory Comparison Study Sluiter, Justin B.; Chum, Helena; Gomes, Absai C. Journal of AOAC INTERNATIONAL, Vol. 99, Issue 3 https://doi.org/10.5740/jaoacint.15-0063	journal	May 2016
CRAFS: a model to analyze two-dimensional X-ray diffraction patterns of plant cellulose Oliveira, Rafael P.; Driemeier, Carlos Journal of Applied Crystallography, Vol. 46, Issue 4 https://doi.org/10.1107/S0021889813014805	journal	July 2013
Dynamic vapor sorption and thermoporometry to probe water in celluloses Driemeier, Carlos; Mendes, Fernanda M.; Oliveira, Marcelo M. Cellulose, Vol. 19, Issue 4 https://doi.org/10.1007/s10570-012-9727-z	journal	May 2012
Absorption of water by polymers: analysis in terms of a simple model Hailwood, A. J.; Horrobin, S. Transactions of the Faraday Society, Vol. 42 https://doi.org/10.1039/tf946420b084	journal	January 1946
Fourier-Transform Raman and Fourier-Transform Infrared Spectroscopy (An Investigation of Five Higher Plant Cell Walls and Their Components) Sene, Cfb.; McCann, M. C.; Wilson, R. H. Plant Physiology, Vol. 106, Issue 4 https://doi.org/10.1104/pp.106.4.1623	journal	December 1994
Improved Xylan Hydrolysis of Corn Stover by Deacetylation with High Solids Dilute Acid Pretreatment Chen, Xiaowen; Shekiro, Joseph; Elander, Rick Industrial & Engineering Chemistry Research, Vol. 51, Issue 1 https://doi.org/10.1021/ie201493g	journal	December 2011
Development and characterization of a high-solids deacetylation process Shekiro, Joseph; Chen, Xiaowen; Smith, Holly Sustainable Chemical Processes, Vol. 4, Issue 1 https://doi.org/10.1186/s40508-016-0049-6	journal	May 2016
Alkaline deacetylation as a strategy to improve sugars recovery and ethanol production from rice straw hemicellulose and cellulose Castro, Rafael Cunha de Assis; Fonseca, Bruno Guedes; dos Santos, Hilton Túlio Lima Industrial Crops and Products, Vol. 106 https://doi.org/10.1016/j.indcrop.2016.08.053	journal	November 2017
Structure of cellulose and microcrystalline cellulose from various wood species, cotton and flax studied by X-ray scattering Leppänen, Kirsi; Andersson, Seppo; Torkkeli, Mika Cellulose, Vol. 16, Issue 6 https://doi.org/10.1007/s10570-009-9298-9	journal	December 2009
Crystallite Width Determines Monolayer Hydration across a Wide Spectrum of Celluloses Isolated from Plants Driemeier, Carlos; Bragatto, Juliano The Journal of Physical Chemistry B, Vol. 117, Issue 1 https://doi.org/10.1021/jp309948h	journal	December 2012
Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates Arantes, Valdeir; Saddler, Jack N. Biotechnology for Biofuels, Vol. 4, Issue 1, Article No. 3 https://doi.org/10.1186/1754-6834-4-3	journal	January 2011
How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility? Ding, S. -Y.; Liu, Y. -S.; Zeng, Y. Science, Vol. 338, Issue 6110 https://doi.org/10.1126/science.1227491	journal	November 2012
Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis Ciesielski, Peter N.; Wang, Wei; Chen, Xiaowen Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-47	journal	January 2014
Assessment of chemical transformations in eucalyptus, sugarcane bagasse and straw during hydrothermal, dilute acid, and alkaline pretreatments Carvalho, Danila Morais de; Sevastyanova, Olena; Penna, Lais Souza Industrial Crops and Products, Vol. 73 https://doi.org/10.1016/j.indcrop.2015.04.021	journal	October 2015
2G ethanol from the whole sugarcane lignocellulosic biomass Pereira, Sandra; Maehara, Larissa; Machado, Cristina Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0224-0	journal	January 2015
Assessment of alkaline pretreatment for the production of bioethanol from eucalyptus, sugarcane bagasse and sugarcane straw Carvalho, Danila Morais de; Queiroz, José Humberto de; Colodette, Jorge Luiz Industrial Crops and Products, Vol. 94 https://doi.org/10.1016/j.indcrop.2016.09.069	journal	December 2016
Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: Enzymatic hydrolysis (part 1) Zeng, Meijuan; Ximenes, Eduardo; Ladisch, Michael R. Biotechnology and Bioengineering, Vol. 109, Issue 2 https://doi.org/10.1002/bit.23337	journal	October 2011
Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment Sun, Qining; Foston, Marcus; Meng, Xianzhi Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0150-6	journal	October 2014
Acetylation of woody lignocellulose: significance and regulation Pawar, Prashant Mohan-Anupama; Koutaniemi, Sanna; Tenkanen, Maija Frontiers in Plant Science, Vol. 4 https://doi.org/10.3389/fpls.2013.00118	journal	January 2013
Determination of cell wall ferulic and p-coumaric acids in sugarcane bagasse Xu, Feng; Sun, Run-Cang; Sun, Jin-Xia Analytica Chimica Acta, Vol. 552, Issue 1-2 https://doi.org/10.1016/j.aca.2005.07.037	journal	November 2005
Hemicelluloses Scheller, Henrik Vibe; Ulvskov, Peter Annual Review of Plant Biology, Vol. 61, Issue 1 https://doi.org/10.1146/annurev-arplant-042809-112315	journal	June 2010
Analysis of soluble and insoluble fractions of alkali and subcritical water treated sugarcane bagasse Ju, Yi-Hsu; Huynh, Lien-Huong; Kasim, Novy Srihartati Carbohydrate Polymers, Vol. 83, Issue 2 https://doi.org/10.1016/j.carbpol.2010.08.022	journal	January 2011
Differences in the chemical structure of the lignins from sugarcane bagasse and straw del Río, José C.; Lino, Alessandro G.; Colodette, Jorge L. Biomass and Bioenergy, Vol. 81 https://doi.org/10.1016/j.biombioe.2015.07.006	journal	October 2015
Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis de Oliveira, Dyoni Matias; Finger-Teixeira, Aline; Rodrigues Mota, Thatiane Plant Biotechnology Journal, Vol. 13, Issue 9 https://doi.org/10.1111/pbi.12292	journal	November 2014
Multiscale deconstruction of molecular architecture in corn stover Inouye, Hideyo; Zhang, Yan; Yang, Lin Scientific Reports, Vol. 4, Issue 1 https://doi.org/10.1038/srep03756	journal	January 2014

Cited By (1)

Intraparticle Connectivity in Sugarcane Bagasse Unveiled by Pore Network Modeling Yancy-Caballero, Daison; Ling, Liu Y.; Fujita, André BioEnergy Research, Vol. 12, Issue 3 https://doi.org/10.1007/s12155-019-09993-6	journal	June 2019