skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Agrobacterium-mediated transformation of lipomyces

Abstract

This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1426818
Patent Number(s):
9,914,932
Application Number:
14/540,818
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Nov 13
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dai, Ziyu, Magnuson, Jon K., Deng, Shuang, Bruno, Kenneth S., and Culley, David E. Agrobacterium-mediated transformation of lipomyces. United States: N. p., 2018. Web.
Dai, Ziyu, Magnuson, Jon K., Deng, Shuang, Bruno, Kenneth S., & Culley, David E. Agrobacterium-mediated transformation of lipomyces. United States.
Dai, Ziyu, Magnuson, Jon K., Deng, Shuang, Bruno, Kenneth S., and Culley, David E. Tue . "Agrobacterium-mediated transformation of lipomyces". United States. https://www.osti.gov/servlets/purl/1426818.
@article{osti_1426818,
title = {Agrobacterium-mediated transformation of lipomyces},
author = {Dai, Ziyu and Magnuson, Jon K. and Deng, Shuang and Bruno, Kenneth S. and Culley, David E.},
abstractNote = {This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {3}
}

Patent:

Save / Share:

Works referenced in this record:

Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure
journal, April 1995

  • Gietz, R. Daniel; Schiestl, Robert H.; Willems, Andrew R.
  • Yeast, Vol. 11, Issue 4, p. 355-360
  • DOI: 10.1002/yea.320110408

Agrobacterium-Mediated Transformation of Non-Plant Organisms
book, January 2008

  • Soltani, Jalal; van Heusden, G. Paul H.; Hooykaas, Paul J. J.
  • Agrobacterium: From Biology to Biotechnology, p. 649-675
  • DOI: 10.1007/978-0-387-72290-0_18

Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.
journal, July 1995


Integration of Agrobacterium tumefaciens T-DNA in the Saccharomyces cerevisiae genome by illegitimate recombination
journal, December 1996

  • Bundock, P.; Hooykaas, P. J. J.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 26, p. 15272-15275
  • DOI: 10.1073/pnas.93.26.15272

Characterization of glyceraldehyde-3-phosphate dehydrogenase gene RtGPD1 and development of genetic transformation method by dominant selection in oleaginous yeast Rhodosporidium toruloides
journal, June 2012

  • Liu, Yanbin; Koh, Chong Mei John; Sun, Longhua
  • Applied Microbiology and Biotechnology, Vol. 97, Issue 2, p. 719-729
  • DOI: 10.1007/s00253-012-4223-9

Review: Plant Binary Vectors of Ti Plasmid in Agrobacterium tumefaciens with a Broad Host-Range Replicon of pRK2, pRi, pSa or pVS1
journal, January 2013


Agrobacterium tumefaciens-mediated transformation of yeast.
journal, February 1996

  • Piers, K. L.; Heath, J. D.; Liang, X.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 4, p. 1613-1618
  • DOI: 10.1073/pnas.93.4.1613

Integration of an insertion-type transferred DNA vector from Agrobacterium tumefaciens into the Saccharomyces cerevisiae genome by gap repair.
journal, October 1996

  • Risseeuw, E.; Franke-van Dijk, M. E.; Hooykaas, P. J.
  • Molecular and Cellular Biology, Vol. 16, Issue 10, p. 5924-5932
  • DOI: 10.1128/MCB.16.10.5924

Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening
journal, January 2012

  • Tapia V, Eulalia; Anschau, Andr�ia; Coradini, Alessandro L. V.
  • AMB Express, Vol. 2, Issue 1, Article No. 64
  • DOI: 10.1186/2191-0855-2-64

Agrobacterium tumefaciens-mediated transformation of filamentous fungi
journal, September 1998

  • de Groot, Marcel J. A.; Bundock, Paul; Hooykaas, Paul J. J.
  • Nature Biotechnology, Vol. 16, Issue 9, p. 839-842
  • DOI: 10.1038/nbt0998-839

Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens.
journal, March 1996


Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool
journal, March 2003


The Initial Steps in Agrobacterium Tumefaciens Pathogenesis: Chemical Biology of Host Recognition
book, January 2008

  • Lin, Yi-Han; Binns, Andrew N.; Lynn, David G.
  • Agrobacterium: From Biology to Biotechnology, p. 221-241
  • DOI: 10.1007/978-0-387-72290-0_6

The Effect of the Agrobacterium tumefaciens attR Mutation on Attachment and Root Colonization Differs between Legumes and Other Dicots
journal, March 2001


Synergistic Action of D-Glucose and Acetosyringone on Agrobacterium Strains for Efficient Dunaliella Transformation
journal, June 2016

  • Srinivasan, Ramachandran; Gothandam, Kodiveri Muthukalianan; Pe�a, Leandro
  • PLOS ONE, Vol. 11, Issue 6, Article No. e0158322
  • DOI: 10.1371/journal.pone.0158322