Agrobacterium-mediated transformation of lipomyces

Dai, Ziyu; Magnuson, Jon K.; Deng, Shuang; Bruno, Kenneth S.; Culley, David E.

Title: Agrobacterium-mediated transformation of lipomyces

Patent · Tue Mar 13 00:00:00 EDT 2018

OSTI ID:1426818

Dai, Ziyu; Magnuson, Jon K.; Deng, Shuang; Bruno, Kenneth S.; Culley, David E.

This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Number(s):: 9,914,932

Application Number:: 14/540,818

OSTI ID:: 1426818

Resource Relation:: Patent File Date: 2014 Nov 13

Country of Publication:: United States

Language:: English

References (20)

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