Effects of humic acid on the interactions between zinc oxide nanoparticles and bacterial biofilms

Ouyang, Kai; Yu, Xiao-Ying; Zhu, Yunlin; Gao, Chunhui; Huang, Qiaoyun; Cai, Peng

doi:10.1016/j.envpol.2017.07.003

Effects of humic acid on the interactions between zinc oxide nanoparticles and bacterial biofilms

Journal Article · 01 December 2017 · Environmental Pollution

DOI:https://doi.org/10.1016/j.envpol.2017.07.003· OSTI ID:1422333

Ouyang, Kai; Yu, Xiao-Ying; Zhu, Yunlin; Gao, Chunhui; Huang, Qiaoyun; Cai, Peng

The effects of humic acid (HA) on interactions between ZnO nanoparticles (ZnO NPs) and Pseudomonas putida KT2440 biofilms at different maturity stages were investigated. Three stages of biofilm development were identified according to bacterial adenosine triphosphate (ATP) activity associated with biofilm development process. In the initial biofilm stage 1, the ATP content of bacteria was reduced by more than 90% when biofilms were exposed to ZnO NPs. However, in the mature biofilm stages 2 and 3, the ATP content was only slightly decreased. Biofilms at stage 3 exhibited less susceptibility to ZnO NPs than biofilms at stage 2. These results suggest that more mature biofilms have a significantly higher tolerance to ZnO NPs compared to young biofilms. In addition, biofilms with intact extracellular poly-meric substances (EPS) showed higher tolerance to ZnO NPs than those without EPS, indicating that EPS play a key role in alleviating the toxic effects of ZnO NPs. In both pure ZnO NPs and ZnO-HA mixtures, dissolved Zn²⁺ originating from the NPs significantly contributed to the overall toxicity. The presence of HA dramatically decreased the toxicity of ZnO NPs due to the binding of Zn²⁺ on HA. The combined results from this work suggest that the biofilm maturity stages and environmental constituents (such as humic acid) are important factors to consider when evaluating potential risks of NPs to ecological systems.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1422333

Report Number(s):: PNNL-SA-132084

Journal Information:: Environmental Pollution, Vol. 231, Issue P1; ISSN 0269-7491

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

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Metabolism, survival, and gene expression of Pseudomonas putida to hematite nanoparticles mediated by surface-bound humic acid No authors listed Environmental Science: Nano https://doi.org/10.1039/c7en01039g		January 2018
Nanotoxicity of engineered nanomaterials (ENMs) to environmentally relevant beneficial soil bacteria – a critical review No authors listed Nanotoxicology https://doi.org/10.1080/17435390.2018.1530391		February 2019
Factors determining the toxicity of engineered nanomaterials to Tetrahymena thermophila in freshwater: the critical role of organic matter No authors listed Environmental Science: Nano https://doi.org/10.1039/c9en01017c		January 2020
Campylobacter jejuni Biofilm Formation Under Aerobic Conditions and Inhibition by ZnO Nanoparticles No authors listed Frontiers in Microbiology https://doi.org/10.3389/fmicb.2020.00207		March 2020

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