Metabolism, survival, and gene expression of Pseudomonas putida to hematite nanoparticles mediated by surface-bound humic acid

Ouyang, Kai; Walker, Sharon L.; Yu, Xiao-Ying; Gao, Chun-Hui; Huang, Qiaoyun; Cai, Peng

doi:10.1039/C7EN01039G

Title: Metabolism, survival, and gene expression of Pseudomonas putida to hematite nanoparticles mediated by surface-bound humic acid

Journal Article · Mon Jan 01 00:00:00 EST 2018 · Environmental Science: Nano

DOI:https://doi.org/10.1039/C7EN01039G· OSTI ID:1439676

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^[1]; Huang, Qiaoyun ^[1];

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State Key Laboratory of Agricultural Microbiology; College of Resources and Environment; Huazhong Agricultural University; Wuhan 430070; China
Department of Chemical and Environmental Engineering; University of California; California 92521; USA
Earth & Biological Sciences Directorate; Pacific Northwest National Laboratory; Richland; USA

Natural organic matter (NOM) is likely to coat naturally occurring nanoparticles (NNPs) in the soil environment and poses distinct effects on the interaction between NPs and soil microorganisms, however such topic has not been well investigated. This study explored the influence of nanoparticle surface-bound humic acid (HA, as a model NOM) on the toxicity of hematite NPs (i.e., nano-Fe2O3) to Pseudomonas putida (P. putida). Results showed that nano-Fe2O3 could inhibit the bacterial growth with an IC50 of 23.58 mg L-1, while nanoparticle surface-bound HA could significantly alleviate the P. putida toxicity of nano-Fe2O3. IC50 of nano-Fe2O3 increased to 4774.23 mg L-1 as a result of surface-saturation by HA. Co-precipitation experiment and transmission electron microscopy observation revealed that nanoparticle surface-bound HA prevented the adhesion of nano-Fe2O3 to the cells as well as limited cell internalization of nanoparticles due to the increased electrostatic repulsion. The generation of intracellular reactive oxygen species (ROS) was significantly limited by the nanoparticle surface-bound HA. The prevention of adhesion and inhibition of ROS generation could account for the HA-mitigated nanotoxicity. Interfacial interactions between hematite NPs and cell membrane were also evaluated on the basis of the Derjaguin–Landau–Verwey–Overbeek (DLVO) theory, and the magnitude of interaction energy barrier correlated well with the 48 h LC50 data of hematite NPs to P. putida. This result implies that metal oxide NPs with strong association with the cell surface might induce more severe cytotoxicity in microorganisms.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1439676

Report Number(s):: PNNL-SA-121317; ESNNA4

Journal Information:: Environmental Science: Nano, Vol. 5, Issue 3; ISSN 2051-8153

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

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Title: Metabolism, survival, and gene expression of Pseudomonas putida to hematite nanoparticles mediated by surface-bound humic acid

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