High-speed fixed-target serial virus crystallography

Roedig, Philip; Ginn, Helen M.; Pakendorf, Tim; Sutton, Geoff; Harlos, Karl; Walter, Thomas S.; Meyer, Jan; Fischer, Pontus; Duman, Ramona; Vartiainen, Ismo; Reime, Bernd; Warmer, Martin; Brewster, Aaron S.; Young, Iris D.; Michels-Clark, Tara; Sauter, Nicholas K.; Kotecha, Abhay; Kelly, James; Rowlands, David J.; Sikorsky, Marcin; Nelson, Silke; Damiani, Daniel S.; Alonso-Mori, Roberto; Ren, Jingshan; Fry, Elizabeth E.; David, Christian; Stuart, David I.; Wagner, Armin; Meents, Alke

doi:10.1038/nmeth.4335

Title: High-speed fixed-target serial virus crystallography

Journal Article · Mon Jun 19 00:00:00 EDT 2017 · Nature Methods

DOI:https://doi.org/10.1038/nmeth.4335· OSTI ID:1421803

Roedig, Philip ^[1]; Ginn, Helen M. ^[2]; Pakendorf, Tim ^[1]; Sutton, Geoff ^[3]; Harlos, Karl ^[3]; Walter, Thomas S. ^[3]; Meyer, Jan ^[1]; Fischer, Pontus ^[1]; Duman, Ramona ^[4]; Vartiainen, Ismo ^[5]; Reime, Bernd ^[1]; Warmer, Martin ^[1]; Brewster, Aaron S. ^[6];

^[6]; Michels-Clark, Tara ^[6]; Sauter, Nicholas K. ^[6]; Kotecha, Abhay ^[3]; Kelly, James ^[7]; Rowlands, David J. ^[8]; Sikorsky, Marcin ^[9] more »

Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science
Univ. of Oxford (United Kingdom). Division of Structural Biology, Wellcome Trust Centre for Human Genetics; Science and Technology Facilities Council (STFC), Harwell Campus, Oxford (United Kingdom). Diamond Light Source, Ltd.
Univ. of Oxford (United Kingdom). Division of Structural Biology, Wellcome Trust Centre for Human Genetics
Science and Technology Facilities Council (STFC), Harwell Campus, Oxford (United Kingdom). Diamond Light Source, Ltd.
Univ. of Eastern Finland, Joensuu (Finland). Inst. of Photonics
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
Univ. of Leeds (United Kingdom). School of Molecular and Cellular Biology; Pirbright Inst., Pirbright (United Kingdom)
Univ. of Leeds (United Kingdom). School of Molecular and Cellular Biology
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Paul Scherrer Inst. (PSI), Villigen (Switzerland)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science; Center for Free Electron Laser Science (CFEL), Hamburg (Germany)

Here, we report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our method allows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); Wellcome Trust

Grant/Contract Number:: AC02-05CH11231; AC02-76SF00515; MR/N00065X/1; GM102520; GM117126

OSTI ID:: 1421803

Journal Information:: Nature Methods, Vol. 14, Issue 8; ISSN 1548-7091

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 81 works

Citation information provided by
Web of Science

References (58)

Goniometer-based femtosecond crystallography with X-ray free electron lasers Cohen, Aina E.; Soltis, S. Michael; González, Ana Proceedings of the National Academy of Sciences, Vol. 111, Issue 48, p. 17122-17127 https://doi.org/10.1073/pnas.1418733111	journal	October 2014
TakeTwo: an indexing algorithm suited to still images with known crystal parameters Ginn, Helen Mary; Roedig, Philip; Kuo, Anling Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 8, p. 956-965 https://doi.org/10.1107/S2059798316010706	journal	July 2016
The molecular organization of cypovirus polyhedra Coulibaly, Fasséli; Chiu, Elaine; Ikeda, Keiko Nature, Vol. 446, Issue 7131 https://doi.org/10.1038/nature05628	journal	March 2007
Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography Mueller, C.; Marx, A.; Epp, S. W. Structural Dynamics, Vol. 2, Issue 5 https://doi.org/10.1063/1.4928706	journal	August 2015
Grease matrix as a versatile carrier of proteins for serial crystallography Sugahara, Michihiro; Mizohata, Eiichi; Nango, Eriko Nature Methods, Vol. 12, Issue 1 https://doi.org/10.1038/nmeth.3172	journal	November 2014
Origin and temperature dependence of radiation damage in biological samples at cryogenic temperatures Meents, A.; Gutmann, S.; Wagner, A. Proceedings of the National Academy of Sciences, Vol. 107, Issue 3 https://doi.org/10.1073/pnas.0905481107	journal	December 2009
Implications for viral uncoating from the structure of bovine enterovirus Smyth, Michael; Tate, John; Hoey, Elizabeth Nature Structural & Molecular Biology, Vol. 2, Issue 3 https://doi.org/10.1038/nsb0395-224	journal	March 1995
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography Boutet, S.; Lomb, L.; Williams, G. J. Science, Vol. 337, Issue 6092 https://doi.org/10.1126/science.1217737	journal	May 2012
Structure of CPV17 polyhedrin determined by the improved analysis of serial femtosecond crystallographic data Ginn, Helen M.; Messerschmidt, Marc; Ji, Xiaoyun Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms7435	journal	March 2015
Femtosecond X-ray protein nanocrystallography Chapman, Henry N.; Fromme, Petra; Barty, Anton Nature, Vol. 470, Issue 7332, p. 73-77 https://doi.org/10.1038/nature09750	journal	February 2011
A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization Walter, Thomas S.; Diprose, Jonathan M.; Mayo, Chris J. Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 6 https://doi.org/10.1107/S0907444905007808	journal	May 2005
Polyhedra structures and the evolution of the insect viruses Ji, Xiaoyun; Axford, Danny; Owen, Robin Journal of Structural Biology, Vol. 192, Issue 1 https://doi.org/10.1016/j.jsb.2015.08.009	journal	October 2015
Preliminary Crystallographic Analysis of Bovine Enterovirus Smyth, Michael; Fry, Elizabeth; Stuart, David Journal of Molecular Biology, Vol. 231, Issue 3 https://doi.org/10.1006/jmbi.1993.1339	journal	June 1993
Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography Oghbaey, Saeed; Sarracini, Antoine; Ginn, Helen M. Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 8, p. 944-955 https://doi.org/10.1107/S2059798316010834	journal	July 2016
Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. Filman, D. J.; Syed, R.; Chow, M. The EMBO Journal, Vol. 8, Issue 5 https://doi.org/10.1002/j.1460-2075.1989.tb03541.x	journal	May 1989
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein Tenboer, Jason; Basu, Shibom; Zatsepin, Nadia Science, Vol. 346, Issue 6214 https://doi.org/10.1126/science.1259357	journal	December 2014
A novel inert crystal delivery medium for serial femtosecond crystallography Conrad, Chelsie E.; Basu, Shibom; James, Daniel IUCrJ, Vol. 2, Issue 4, p. 421-430 https://doi.org/10.1107/S2052252515009811	journal	June 2015
Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser Kupitz, Christopher; Basu, Shibom; Grotjohann, Ingo Nature, Vol. 513, Issue 7517 https://doi.org/10.1038/nature13453	journal	July 2014
Crystallizing membrane proteins using lipidic mesophases Caffrey, Martin; Cherezov, Vadim Nature Protocols, Vol. 4, Issue 5 https://doi.org/10.1038/nprot.2009.31	journal	April 2009
Bovine enterovirus 2: complete genomic sequence and molecular modelling of a reference strain and a wild-type isolate from endemically infected US cattle Goens, S. D. Journal of General Virology, Vol. 85, Issue 11 https://doi.org/10.1099/vir.0.80159-0	journal	November 2004
Virus Crystallography Fry, Elizabeth E.; Grimes, Jonathan; Stuart, David I. Molecular Biotechnology, Vol. 12, Issue 1 https://doi.org/10.1385/MB:12:1:13	journal	January 1999
Potential for biomolecular imaging with femtosecond X-ray pulses Neutze, Richard; Wouts, Remco; van der Spoel, David Nature, Vol. 406, Issue 6797 https://doi.org/10.1038/35021099	journal	August 2000
Measurement of the equilibrium relative humidity for common precipitant concentrations: facilitating controlled dehydration experiments Wheeler, Matthew J.; Russi, Silvia; Bowler, Michael G. Acta Crystallographica Section F Structural Biology and Crystallization Communications, Vol. 68, Issue 1 https://doi.org/10.1107/S1744309111054029	journal	December 2011
Serial Femtosecond Crystallography of G Protein-Coupled Receptors Liu, W.; Wacker, D.; Gati, C. Science, Vol. 342, Issue 6165 https://doi.org/10.1126/science.1244142	journal	December 2013
Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements Barty, Anton; Caleman, Carl; Aquila, Andrew Nature Photonics, Vol. 6, Issue 1 https://doi.org/10.1038/nphoton.2011.297	journal	December 2011
Low-dose fixed-target serial synchrotron crystallography Owen, Robin L.; Axford, Danny; Sherrell, Darren A. Acta Crystallographica Section D Structural Biology, Vol. 73, Issue 4, p. 373-378 https://doi.org/10.1107/S2059798317002996	journal	March 2017
Closing the door on flaviviruses: Entry as a target for antiviral drug design Perera, Rushika; Khaliq, Mansoora; Kuhn, Richard J. Antiviral Research, Vol. 80, Issue 1 https://doi.org/10.1016/j.antiviral.2008.05.004	journal	October 2008
Role of Electrostatic Repulsion in Controlling pH-Dependent Conformational Changes of Viral Fusion Proteins Harrison, Joseph S.; Higgins, Chelsea D.; O’Meara, Matthew J. Structure, Vol. 21, Issue 7 https://doi.org/10.1016/j.str.2013.05.009	journal	July 2013
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography Weierstall, Uwe; James, Daniel; Wang, Chong Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4309	journal	February 2014
Fixed-target protein serial microcrystallography with an x-ray free electron laser Hunter, Mark S.; Segelke, Brent; Messerschmidt, Marc Scientific Reports, Vol. 4, Issue 1 https://doi.org/10.1038/srep06026	journal	August 2014
On the release of cppxfel for processing X-ray free-electron laser images Ginn, Helen Mary; Evans, Gwyndaf; Sauter, Nicholas K. Journal of Applied Crystallography, Vol. 49, Issue 3 https://doi.org/10.1107/S1600576716006981	journal	May 2016
High-density grids for efficient data collection from multiple crystals Baxter, Elizabeth L.; Aguila, Laura; Alonso-Mori, Roberto Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 1, p. 2-11 https://doi.org/10.1107/S2059798315020847	journal	January 2016
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan Journal of Applied Crystallography, Vol. 49, Issue 3 https://doi.org/10.1107/S1600576716006348	journal	May 2016
Fusing structure and function: a structural view of the herpesvirus entry machinery Connolly, Sarah A.; Jackson, Julia O.; Jardetzky, Theodore S. Nature Reviews Microbiology, Vol. 9, Issue 5 https://doi.org/10.1038/nrmicro2548	journal	April 2011
Version 1.2 of the Crystallography and NMR system Brunger, Axel T. Nature Protocols, Vol. 2, Issue 11 https://doi.org/10.1038/nprot.2007.406	journal	October 2007
Cryocrystallography of biological macromolecules: a generally applicable method Hope, H. Acta Crystallographica Section B Structural Science, Vol. 44, Issue 1 https://doi.org/10.1107/S0108768187008632	journal	February 1988
Sample Preparation and Data Collection for High-Speed Fixed-Target Serial Femtosecond Crystallography Meents, Alke; Roedig, Philip; Ginn, Helen M. Protocol Exchange https://doi.org/10.1038/protex.2017.059	journal	June 2017
A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering Roedig, P.; Vartiainen, I.; Duman, R. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep10451	journal	May 2015
A Sialic Acid Binding Site in a Human Picornavirus Zocher, Georg; Mistry, Nitesh; Frank, Martin PLoS Pathogens, Vol. 10, Issue 10 https://doi.org/10.1371/journal.ppat.1004401	journal	October 2014
Serial femtosecond crystallography of soluble proteins in lipidic cubic phase Fromme, Raimund; Ishchenko, Andrii; Metz, Markus IUCrJ, Vol. 2, Issue 5, p. 545-551 https://doi.org/10.1107/S2052252515013160	journal	August 2015
Experimental determination of the radiation dose limit for cryocooled protein crystals Owen, R. L.; Rudino-Pinera, E.; Garman, E. F. Proceedings of the National Academy of Sciences, Vol. 103, Issue 13 https://doi.org/10.1073/pnas.0600973103	journal	March 2006
Crystal lattice as biological phenotype for insect viruses Anduleit, Karin; Sutton, Geoff; Diprose, Jonathan M. Protein Science, Vol. 14, Issue 10 https://doi.org/10.1110/ps.051516405	journal	October 2005
In situ macromolecular crystallography using microbeams Axford, Danny; Owen, Robin L.; Aishima, Jun Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 5 https://doi.org/10.1107/S0907444912006749	journal	April 2012
A modular and compact portable mini-endstation for high-precision, high-speed fixed target serial crystallography at FEL and synchrotron sources Sherrell, Darren A.; Foster, Andrew J.; Hudson, Lee Journal of Synchrotron Radiation, Vol. 22, Issue 6 https://doi.org/10.1107/S1600577515016938	journal	October 2015
How baculovirus polyhedra fit square pegs into round holes to robustly package viruses Ji, Xiaoyun; Sutton, Geoff; Evans, Gwyndaf The EMBO Journal, Vol. 29, Issue 2 https://doi.org/10.1038/emboj.2009.352	journal	December 2009
Macromolecular diffractive imaging using imperfect crystals Ayyer, Kartik; Yefanov, Oleksandr M.; Oberthür, Dominik Nature, Vol. 530, Issue 7589, p. 202-206 https://doi.org/10.1038/nature16949	journal	February 2016
Serial femtosecond X-ray diffraction of enveloped virus microcrystals Lawrence, Robert M.; Conrad, Chelsie E.; Zatsepin, Nadia A. Structural Dynamics, Vol. 2, Issue 4 https://doi.org/10.1063/1.4929410	journal	July 2015
Gas dynamic virtual nozzle for generation of microscopic droplet streams DePonte, D. P.; Weierstall, U.; Schmidt, K. Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505 https://doi.org/10.1088/0022-3727/41/19/195505	journal	September 2008
Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser Redecke, L.; Nass, K.; DePonte, D. P. Science, Vol. 339, Issue 6116 https://doi.org/10.1126/science.1229663	journal	November 2012
Oil-free hyaluronic acid matrix for serial femtosecond crystallography Sugahara, Michihiro; Song, Changyong; Suzuki, Mamoru Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep24484	journal	April 2016
Macromolecular diffractive imaging using imperfect crystals Ayyer, Kartik; Yefanov, Oleksandr; Oberthür, Dominik Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2016-01028	text	January 2016
Room-Temperature Macromolecular Crystallography Using a Micro-Patterned Silicon Chip with Minimal Background Scattering Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2016-02690	text	January 2016
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
Role of Electrostatic Repulsion in Controlling pH-Dependent Conformational Changes of Viral Fusion Proteins D., Higgins, Chelsea; S., Harrison, Joseph; F., Koellhoffer, Jayne The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/wzdz-sn06	text	January 2013
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
Serial femtosecond crystallography of soluble proteins in lipidic cubic phase Fromme, Raimund; Ishchenko, Andrii; Metz, Markus Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2015-04280	text	January 2015
The Highly Ordered Double-Stranded RNA Genome of Bluetongue Virus Revealed by Crystallography Gouet, Patrice; Diprose, Jonathan M.; Grimes, Jonathan M. Cell, Vol. 97, Issue 4 https://doi.org/10.1016/s0092-8674(00)80758-8	journal	May 1999

Cited By (45)

Stimulated transitions in resonant atom Majorana mixing Bernabéu, José; Segarra, Alejandro Journal of High Energy Physics, Vol. 2018, Issue 2 https://doi.org/10.1007/jhep02(2018)017	journal	February 2018
Multi-wavelength anomalous diffraction de novo phasing using a two-colour X-ray free-electron laser with wide tunability Gorel, Alexander; Motomura, Koji; Fukuzawa, Hironobu Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-00754-7	journal	October 2017
Pink-beam serial crystallography Meents, A.; Wiedorn, M. O.; Srajer, V. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01417-3	journal	November 2017
Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14434-6	journal	January 2020
The life cycle of non-polio enteroviruses and how to target it Baggen, Jim; Thibaut, Hendrik Jan; Strating, Jeroen R. P. M. Nature Reviews Microbiology, Vol. 16, Issue 6 https://doi.org/10.1038/s41579-018-0005-4	journal	April 2018
Towards in cellulo virus crystallography Duyvesteyn, Helen M. E.; Ginn, Helen M.; Pietilä, Maija K. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-21693-3	journal	February 2018
Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography Lee, Donghyeon; Baek, Sangwon; Park, Jaehyun Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-43485-z	journal	May 2019
Glutathione facilitates enterovirus assembly by binding at a druggable pocket Duyvesteyn, Helen M. E.; Ren, Jingshan; Walter, Thomas S. Communications Biology, Vol. 3, Issue 1 https://doi.org/10.1038/s42003-019-0722-x	journal	January 2020
Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; van Bokhoven, Jeroen A. Structural Dynamics, Vol. 4, Issue 6 https://doi.org/10.1063/1.4997222	journal	November 2017
An outlook on using serial femtosecond crystallography in drug discovery Mishin, Alexey; Gusach, Anastasiia; Luginina, Aleksandra Expert Opinion on Drug Discovery, Vol. 14, Issue 9 https://doi.org/10.1080/17460441.2019.1626822	journal	May 2019
X-ray free-electron laser: opportunities for drug discovery Cheng, Robert; Abela, Rafael; Hennig, Michael Acta Crystallographica Section A Foundations and Advances, Vol. 74, Issue a1 https://doi.org/10.1107/s0108767318095570	journal	July 2018
Protein crystals IR laser ablated from aqueous solution at high speed retain their diffractive properties: applications in high-speed serial crystallography Schulz, Eike C.; Kaub, Johannes; Busse, Frederik Journal of Applied Crystallography, Vol. 50, Issue 6 https://doi.org/10.1107/s1600576717014479	journal	November 2017
3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating Echelmeier, Austin; Kim, Daihyun; Cruz Villarreal, Jorvani Journal of Applied Crystallography, Vol. 52, Issue 5 https://doi.org/10.1107/s1600576719010343	journal	August 2019
DatView : a graphical user interface for visualizing and querying large data sets in serial femtosecond crystallography Stander, Natasha; Fromme, Petra; Zatsepin, Nadia Journal of Applied Crystallography, Vol. 52, Issue 6 https://doi.org/10.1107/s1600576719012044	journal	October 2019
Successful sample preparation for serial crystallography experiments Beale, John H.; Bolton, Rachel; Marshall, Stephen A. Journal of Applied Crystallography, Vol. 52, Issue 6 https://doi.org/10.1107/s1600576719013517	journal	November 2019
The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source Sierra, Raymond G.; Batyuk, Alexander; Sun, Zhibin Journal of Synchrotron Radiation, Vol. 26, Issue 2 https://doi.org/10.1107/s1600577519001577	journal	February 2019
Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals Casadei, Cecilia M.; Nass, Karol; Barty, Anton IUCrJ, Vol. 6, Issue 1 https://doi.org/10.1107/s2052252518014641	journal	January 2019
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan IUCrJ, Vol. 6, Issue 4 https://doi.org/10.1107/s2052252519007395	journal	June 2019
Toward G protein-coupled receptor structure-based drug design using X-ray lasers Ishchenko, Andrii; Stauch, Benjamin; Han, Gye Won IUCrJ, Vol. 6, Issue 6 https://doi.org/10.1107/s2052252519013137	journal	October 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L.; Gilbile, D.; Grant, T. D. IUCrJ, Vol. 7, Issue 1 https://doi.org/10.1107/s2052252519014003	journal	January 2020
Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser Jones, Christopher; Tran, Brandon; Conrad, Chelsie Acta Crystallographica Section F Structural Biology Communications, Vol. 75, Issue 7 https://doi.org/10.1107/s2053230x19008549	journal	June 2019
Co-crystal structure of the i Mango-III fluorescent RNA aptamer using an X-ray free-electron laser Trachman, Robert J.; Stagno, Jason R.; Conrad, Chelsie Acta Crystallographica Section F Structural Biology Communications, Vol. 75, Issue 8 https://doi.org/10.1107/s2053230x19010136	journal	August 2019
Where is crystallography going? Grimes, Jonathan M.; Hall, David R.; Ashton, Alun W. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 2 https://doi.org/10.1107/s2059798317016709	journal	February 2018
ZOO : an automatic data-collection system for high-throughput structure analysis in protein microcrystallography Hirata, Kunio; Yamashita, Keitaro; Ueno, Go Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017795	journal	January 2019
A guide to sample delivery systems for serial crystallography Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai The FEBS Journal, Vol. 286, Issue 22 https://doi.org/10.1111/febs.15099	journal	August 2019
Protein phase separation and determinants of in cell crystallization Mudogo, Celestin N.; Falke, Sven; Brognaro, Hévila Traffic, Vol. 21, Issue 2 https://doi.org/10.1111/tra.12711	journal	November 2019
Protein crystallization in living cells Schönherr, Robert; Rudolph, Janine Mia; Redecke, Lars Biological Chemistry, Vol. 399, Issue 7 https://doi.org/10.1515/hsz-2018-0158	journal	June 2018
Sample Delivery Media for Serial Crystallography Nam, Ki International Journal of Molecular Sciences, Vol. 20, Issue 5 https://doi.org/10.3390/ijms20051094	journal	March 2019
Polysaccharide-Based Injection Matrix for Serial Crystallography Nam, Ki Hyun International Journal of Molecular Sciences, Vol. 21, Issue 9 https://doi.org/10.3390/ijms21093332	journal	May 2020
X-ray free electron laser: opportunities for drug discovery Cheng, Robert K. Y.; Abela, Rafael; Hennig, Michael Essays in Biochemistry, Vol. 61, Issue 5 https://doi.org/10.1042/ebc20170031	journal	November 2017
High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner Gao, Yuan; Xu, Weihe; Shi, Wuxian International Union of Crystallography (IUCr) https://doi.org/10.1107/s1600577518010354/yn5033sup1.pdf	text	January 2018
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan International Union of Crystallography (IUCr) https://doi.org/10.1107/s2052252519007395/mf5034sup1.pdf	text	January 2019
Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals Casadei, Cecilia M.; Nass, Karol; Barty, Anton Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-04823	text	January 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L.; Gilbile, D.; Grant, T. D. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-05316	text	January 2020
Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00687	text	January 2020
Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; Van Bokhoven, Jeroen A. AIP Publishing https://doi.org/10.7892/boris.114739	text	January 2017
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation Mancuso, Adrian P.; Aquila, Andrew; Batchelor, Lewis Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-01957	text	January 2019
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-02620	text	January 2019
Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser Jones, Christopher; Tran, Brandon; Conrad, Chelsie Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-02740	text	January 2019
Protein phase separation and determinants of in cell crystallization Mudogo, Celestin Nzanzu; Falke, Sven; Brognaro dos Santos, Hevila Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00138	text	January 2019
Ultracompact 3D microfluidics for time-resolved structural biology Knoska, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00623	text	January 2020
Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; Van Bokhoven, Jeroen A. ETH Zurich https://doi.org/10.3929/ethz-b-000231826	text	January 2017
Stimulated transitions in resonant atom Majorana mixing Bernabeu, Jose; Segarra, Alejandro arXiv https://doi.org/10.48550/arxiv.1706.08328	text	January 2017
Lard Injection Matrix for Serial Crystallography Nam, Ki Hyun International Journal of Molecular Sciences, Vol. 21, Issue 17 https://doi.org/10.3390/ijms21175977	journal	August 2020

Similar Records

Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals

Journal Article · Fri Jun 18 00:00:00 EDT 2021 · IUCrJ · OSTI ID:1421803

Lahey-Rudolph, J. Mia; Schönherr, Robert; Barthelmess, Miriam; +5 more

Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography

Journal Article · Thu Nov 25 00:00:00 EST 2021 · Lab on a Chip · OSTI ID:1421803

Gilbile, Deepshika; Shelby, Megan L.; Lyubimov, Artem Y.; +7 more

Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Journal Article · Wed Feb 26 00:00:00 EST 2020 · IUCrJ · OSTI ID:1421803

Wolff, Alexander M.; Young, Iris D.; Sierra, Raymond G.; +35 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Nanocrystallography
Proteins
Virology

Title: High-speed fixed-target serial virus crystallography

Citation Formats

References (58)

Cited By (45)

Similar Records

Related Subjects