Protein-Containing Lipid Bilayers Intercalated with Size-Matched Mesoporous Silica Thin Films

Isaksson, Simon; Watkins, Erik Benjamin; Browning, Kathryn L.; Lind, Tania Kjellerup; Cardenas, Marite; Hedfalk, Kristina; Hook, Fredrik; Andersson, Martin

doi:10.1021/acs.nanolett.6b04493

Protein-Containing Lipid Bilayers Intercalated with Size-Matched Mesoporous Silica Thin Films

Journal Article · Tue Nov 22 23:00:00 EST 2016 · Nano Letters

DOI:https://doi.org/10.1021/acs.nanolett.6b04493· OSTI ID:1418770

Isaksson, Simon ^[1]; ^[2]; Browning, Kathryn L. ^[3]; Lind, Tania Kjellerup ^[4]; Cardenas, Marite ^[4]; Hedfalk, Kristina ^[5]; Hook, Fredrik ^[6]; ^[1]

Chambers Univ. of Technology, Gothenburg (Sweden)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Uppsala Univ., Uppsala (Sweden)
Malmo Univ., Malmo (Sweden)
Univ. of Gothenburg, Gothenburg (Sweden)
Chalmers Univ. of Technology, Gothenburg (Sweden)

Here, proteins are key components in a multitude of biological processes, of which the functions carried out by transmembrane (membrane-spanning) proteins are especially demanding for investigations. This is because this class of protein needs to be incorporated into a lipid bilayer representing its native environment, and in addition, many experimental conditions also require a solid support for stabilization and analytical purposes. The solid support substrate may, however, limit the protein functionality due to protein–material interactions and a lack of physical space. We have in this work tailored the pore size and pore ordering of a mesoporous silica thin film to match the native cell-membrane arrangement of the transmembrane protein human aquaporin 4 (hAQP4). Using neutron reflectivity (NR), we provide evidence of how substrate pores host the bulky water-soluble domain of hAQP4, which is shown to extend 7.2 nm into the pores of the substrate. Complementary surface analytical tools, including quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy, revealed successful protein-containing supported lipid bilayer (pSLB) formation on mesoporous silica substrates, whereas pSLB formation was hampered on nonporous silica. Additionally, electron microscopy (TEM and SEM), light scattering (DLS and stopped-flow), and small-angle X-ray scattering (SAXS) were employed to provide a comprehensive characterization of this novel hybrid organic–inorganic interface, the tailoring of which is likely to be generally applicable to improve the function and stability of a broad range of membrane proteins containing water-soluble domains.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: Universities/Institutions; USDOE

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1418770

Report Number(s):: LA-UR--17-28188

Journal Information:: Nano Letters, Journal Name: Nano Letters Journal Issue: 1 Vol. 17; ISSN 1530-6984

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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An Assessment of the Use of Mesoporous Silica Materials to Improve Pulsed Dipolar Spectroscopy Lai, Yei-Chen; Chang, Albert; Yang, Chia-Min Applied Magnetic Resonance, Vol. 49, Issue 11 https://doi.org/10.1007/s00723-018-1040-z	journal	July 2018
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59 BASIC BIOLOGICAL SCIENCES
Aquaporin
Lipid bilayer
Liposome
Membrane protein
Neutron reflectivity
Silica
lipids aquaporin silica

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