Significance of Lignin S/G Ratio in Biomass Recalcitrance of Populus trichocarpa Variants for Bioethanol Production

Yoo, Chang Geun; Dumitrache, Alexandru; Muchero, Wellington; Natzke, Jace; Akinosho, Hannah; Li, Mi; Sykes, Robert W.; Brown, Steven D.; Davison, Brian; Tuskan, Gerald A.; Pu, Yunqiao; Ragauskas, Arthur J.

doi:10.1021/acssuschemeng.7b03586

Significance of Lignin S/G Ratio in Biomass Recalcitrance of Populus trichocarpa Variants for Bioethanol Production

Journal Article · Sun Dec 10 23:00:00 EST 2017 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.7b03586· OSTI ID:1415909

Yoo, Chang Geun ^[1]; Dumitrache, Alexandru ^[1]; Muchero, Wellington ^[1]; Natzke, Jace ^[1]; Akinosho, Hannah ^[2]; Li, Mi ^[1]; Sykes, Robert W. ^[3]; Brown, Steven D. ^[1]; Davison, Brian ^[1]; Tuskan, Gerald A. ^[1]; Pu, Yunqiao ^[1]; ^[4]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Georgia Inst. of Technology, Atlanta, GA (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Lignin S/G ratio has been investigated as an important factor in biomass recalcitrance to bioethanol production. Because of the complexity and variety of biomass, recalcitrance was also reportedly influenced by several other factors, such as total lignin content, degree of cellulose polymerization, etc. In addition, the effect of S/G ratio on biomass conversion is not uniform across plant species. Herein, 11 Populus trichocarpa natural variants grown under the same conditions with similar total lignin content were selected to minimize the effects of other factors. The lignin S/G ratio of the selected P. trichocarpa natural variants showed negative correlations with p-hydroxybenzoate (PB) and β–5 linkage contents, while it had positive ones with β-O-4 linkage, lignin molecular weight, and ethanol production. In conclusion, this study showed the importance of lignin S/G ratio as an independent recalcitrance factor that may aid future energy crop engineering and biomass conversion strategies.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1415909

Journal Information:: ACS Sustainable Chemistry & Engineering, Journal Name: ACS Sustainable Chemistry & Engineering Journal Issue: 2 Vol. 6; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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