An Automated Microfluidic Assay for Photonic Crystal Enhanced Detection and Analysis of an Antiviral Antibody Cancer Biomarker in Serum

Journal Article · Fri Nov 24 00:00:00 EST 2017 · IEEE Sensors Journal

DOI:https://doi.org/10.1109/JSEN.2017.2777529· OSTI ID:1413458

Race, Caitlin M. ^[1]; Kwon, Lydia E. ^[2]; Foreman, Myles T. ^[1]; Huang, Qinglan ^[1]; Inan, Hakan ^[3]; Kesiraju, Sailaja ^[4]; Le, Phuong ^[5]; Lim, Sung Jun ^[5]; Smith, Andrew M. ^[5]; Zangar, Richard C. ^[6]; Demirci, Utkan ^[3]; Anderson, Karen S. ^[4]; Cunningham, Brian T. ^[7]

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1. Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Electrical and Computer Engineering
2. Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Bioengineering; Univ. of Illinois, Urbana-Champaign, IL (United States). College of Medicine
3. Stanford Univ., CA (United States). Canary Center for Cancer Early Detection
4. Arizona State Univ., Tempe, AZ (United States). Biodesign Inst.
5. Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Bioengineering
6. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
7. Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Electrical and Computer Engineering; Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Bioengineering

Here, we report on the implementation of an automated platform for detecting the presence of an antibody biomarker for human papillomavirus-associated oropharyngeal cancer from a single droplet of serum, in which a nanostructured photonic crystal surface is used to amplify the output of a fluorescence-linked immunosorbent assay. The platform is comprised of a microfluidic cartridge with integrated photonic crystal chips that interfaces with an assay instrument that automates the introduction of reagents, wash steps, and surface drying. Upon assay completion, the cartridge interfaces with a custom laser-scanning instrument that couples light into the photonic crystal at the optimal resonance condition for fluorescence enhancement. The instrument is used to measure the fluorescence intensity values of microarray spots corresponding to the biomarkers of interest, in addition to several experimental controls that verify correct functioning of the assay protocol. In this work, we report both dose-response characterization of the system using anti-E7 antibody introduced at known concentrations into serum and characterization of a set of clinical samples from which results were compared with a conventional enzyme-linked immunosorbent assay (ELISA) performed in microplate format. Finally, the demonstrated capability represents a simple, rapid, automated, and high-sensitivity method for multiplexed detection of protein biomarkers from a low-volume test sample.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE; National Institutes of Health (NIH)
• Grant/Contract Number: AC05-76RL01830; 5R33CA177446-02; 5R01GM086382-03
• OSTI ID: 1413458
• Journal Information: IEEE Sensors Journal, Vol. 18, Issue 4; ISSN 1530-437X
• Publisher: IEEE
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Proteins
Cancer
Fluorescence
Microfluidics
Photonic crystals
Immune system
Instruments
automated
assay
biomarker
cancer
E7
fluorescence
human papillomavirus
immunoassay
microfluidic
oropharyngeal cancer
photonic crystal
point-of-care
serum