Multimodal hyperspectral optical microscopy

Journal Article · Sat Sep 02 00:00:00 EDT 2017 · Chemical Physics

DOI:https://doi.org/10.1016/j.chemphys.2017.08.011· OSTI ID:1411908

Novikova, Irina V. ^[1]; Smallwood, Chuck R. ^[1]; Gong, Yu ^[2]; Hu, Dehong ^[1]; Hendricks, Leif ^[3]; Evans, James E. ^[1]; Bhattarai, Ashish ^[2]; Hess, Wayne P. ^[2]; El-Khoury, Patrick Z. ^[2]

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1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Physical Sciences Div.
3. Surface Optics Corp., San Diego, CA (United States)

We describe a unique and convenient approach to multimodal hyperspectral optical microscopy, herein achieved by coupling a portable and transferable hyperspectral imager to various optical microscopes. The experimental and data analysis schemes involved in recording spectrally and spatially resolved fluorescence, dark field, and optical absorption micrographs are illustrated through prototypical measurements targeting selected model systems. Namely, hyperspectral fluorescence micrographs of isolated fluorescent beads are employed to ensure spectral calibration of our detector and to gauge the attainable spatial resolution of our measurements; the recorded images are diffraction-limited. Moreover, spatially over-sampled absorption spectroscopy of a single lipid (18:1 Liss Rhod PE) layer reveals that optical densities on the order of 10-3 may be resolved by spatially averaging the recorded optical signatures. We also briefly illustrate two applications of our setup in the general areas of plasmonics and cell biology. Most notably, we deploy hyperspectral optical absorption microscopy to identify and image algal pigments within a single live Tisochrysis lutea cell. Overall, this work paves the way for multimodal multidimensional spectral imaging measurements spanning the realms of several scientific disciples.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
• Grant/Contract Number: AC05-76RL01830
• OSTI ID: 1411908
• Alternate ID(s): OSTI ID: 1495725
• Report Number(s): PNNL-SA--120193; 49643; KC0301050; KP1606000; KP1606000
• Journal Information: Chemical Physics, Journal Name: Chemical Physics Vol. 498-499; ISSN 0301-0104
• Publisher: Elsevier
• Country of Publication: United States
• Language: English

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