Nanocrystallography measurements of early stage synthetic malaria pigment

Dilanian, Ruben A.; Streltsov, Victor; Coughlan, Hannah D.; Quiney, Harry M.; Martin, Andrew V.; Klonis, Nectarios; Dogovski, Con; Boutet, Sébastien; Messerschmidt, Marc; Williams, Garth J.; Williams, Sophie; Phillips, Nicholas W.; Nugent, Keith A.; Tilley, Leann; Abbey, Brian

doi:10.1107/s1600576717012663

Nanocrystallography measurements of early stage synthetic malaria pigment

Journal Article · Thu Sep 28 00:00:00 EDT 2017 · Journal of Applied Crystallography (Online)

DOI:https://doi.org/10.1107/s1600576717012663· OSTI ID:1407496

Dilanian, Ruben A. ^[1]; Streltsov, Victor ^[2]; ^[3]; Quiney, Harry M. ^[1]; Martin, Andrew V. ^[1]; Klonis, Nectarios ^[4]; Dogovski, Con ^[4]; ^[5]; ^[6]; Williams, Garth J. ^[7]; Williams, Sophie ^[1]; Phillips, Nicholas W. ^[8]; ^[9]; Tilley, Leann ^[4]; ^[9]

Univ. of Melbourne (Australia). ARC Centre of Excellenece in Advanced Molecular Imaging
Commonwealth Scientific and Industrial Research Organization (CSIRO), Parkville VIC (Australia)
La Trobe Univ., Melbourne, VIC (Australia). ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Inst. of Medical Research; Walter and Eliza Hall Inst. of Medical Research, Parkville, VIC (Australia); Commonwealth Scientific and Industrial Research Organization (CSIRO) Manufacturing Flagship, Parkville VIC (Australia)
Univ. of Melbourne (Australia). Dept. of Biochemistry and Molecular Biology, Molecular Science and Biotechnology Inst.
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
BioXFEL STC Buffalo, NY (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)
Commonwealth Scientific and Industrial Research Organization (CSIRO), Parkville VIC (Australia); La Trobe Univ., Melbourne, VIC (Australia). ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Inst. of Medical Research
La Trobe Univ., Melbourne, VIC (Australia). ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Inst. of Medical Research

The recent availability of extremely intense, femtosecond X-ray free-electron laser (XFEL) sources has spurred the development of serial femtosecond nanocrystallography (SFX). Here, SFX is used to analyze nanoscale crystals of β-hematin, the synthetic form of hemozoin which is a waste by-product of the malaria parasite. This analysis reveals significant differences in β-hematin data collected during SFX and synchrotron crystallography experiments. To interpret these differences two possibilities are considered: structural differences between the nanocrystal and larger crystalline forms of β-hematin, and radiation damage. Simulation studies show that structural inhomogeneity appears at present to provide a better fit to the experimental data. If confirmed, these observations will have implications for designing compounds that inhibit hemozoin formation and suggest that, for some systems at least, additional information may be gained by comparing structures obtained from nanocrystals and macroscopic crystals of the same molecule.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1407496

Alternate ID(s):: OSTI ID: 1418317
OSTI ID: 1433955

Journal Information:: Journal of Applied Crystallography (Online), Journal Name: Journal of Applied Crystallography (Online) Journal Issue: 5 Vol. 50; ISSN 1600-5767; ISSN JACGAR

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Nanocrystallography measurements of early stage synthetic malaria pigment

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