Nanocrystallography measurements of early stage synthetic malaria pigment

Dilanian, Ruben A.; Streltsov, Victor; Coughlan, Hannah D.; Quiney, Harry M.; Martin, Andrew V.; Klonis, Nectarios; Dogovski, Con; Boutet, Sébastien; Messerschmidt, Marc; Williams, Garth J.; Williams, Sophie; Phillips, Nicholas W.; Nugent, Keith A.; Tilley, Leann; Abbey, Brian

doi:10.1107/s1600576717012663

Title: Nanocrystallography measurements of early stage synthetic malaria pigment

Journal Article · Thu Sep 28 00:00:00 EDT 2017 · Journal of Applied Crystallography (Online)

DOI:https://doi.org/10.1107/s1600576717012663· OSTI ID:1407496

Dilanian, Ruben A. ^[1]; Streltsov, Victor ^[2];

^[3]; Quiney, Harry M. ^[1]; Martin, Andrew V. ^[1]; Klonis, Nectarios ^[4]; Dogovski, Con ^[4];

^[5];

^[6]; Williams, Garth J. ^[7]; Williams, Sophie ^[1]; Phillips, Nicholas W. ^[8];

^[9]; Tilley, Leann ^[4];

^[9]

Univ. of Melbourne (Australia). ARC Centre of Excellenece in Advanced Molecular Imaging
Commonwealth Scientific and Industrial Research Organization (CSIRO), Parkville VIC (Australia)
La Trobe Univ., Melbourne, VIC (Australia). ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Inst. of Medical Research; Walter and Eliza Hall Inst. of Medical Research, Parkville, VIC (Australia); Commonwealth Scientific and Industrial Research Organization (CSIRO) Manufacturing Flagship, Parkville VIC (Australia)
Univ. of Melbourne (Australia). Dept. of Biochemistry and Molecular Biology, Molecular Science and Biotechnology Inst.
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
BioXFEL STC Buffalo, NY (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)
Commonwealth Scientific and Industrial Research Organization (CSIRO), Parkville VIC (Australia); La Trobe Univ., Melbourne, VIC (Australia). ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Inst. of Medical Research
La Trobe Univ., Melbourne, VIC (Australia). ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Inst. of Medical Research

The recent availability of extremely intense, femtosecond X-ray free-electron laser (XFEL) sources has spurred the development of serial femtosecond nanocrystallography (SFX). Here, SFX is used to analyze nanoscale crystals of β-hematin, the synthetic form of hemozoin which is a waste by-product of the malaria parasite. This analysis reveals significant differences in β-hematin data collected during SFX and synchrotron crystallography experiments. To interpret these differences two possibilities are considered: structural differences between the nanocrystal and larger crystalline forms of β-hematin, and radiation damage. Simulation studies show that structural inhomogeneity appears at present to provide a better fit to the experimental data. If confirmed, these observations will have implications for designing compounds that inhibit hemozoin formation and suggest that, for some systems at least, additional information may be gained by comparing structures obtained from nanocrystals and macroscopic crystals of the same molecule.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515; SC0012704

OSTI ID:: 1407496

Alternate ID(s):: OSTI ID: 1418317; OSTI ID: 1433955

Report Number(s):: BNL-203476-2018-JAAM; JACGAR; PII: S1600576717012663; TRN: US1703051

Journal Information:: Journal of Applied Crystallography (Online), Vol. 50, Issue 5; ISSN 1600-5767

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 10 works

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Related Subjects

60 APPLIED LIFE SCIENCES
59 BASIC BIOLOGICAL SCIENCES
crystalloraphy
serial femtosecond nanocrystallography
malaria
crystalline disorder
structural inhomogeniety
36 MATERIALS SCIENCE
crystallography