Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles

Paxton, Walter F.; McAninch, Patrick T.; Achyuthan, Komandoor E.; Shin, Sun Hae Ra; Monteith, Haley L.

doi:10.1016/j.colsurfb.2017.07.091

Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles

Journal Article · Tue Aug 01 00:00:00 EDT 2017 · Colloids and Surfaces. B, Biointerfaces

DOI:https://doi.org/10.1016/j.colsurfb.2017.07.091· OSTI ID:1399496

Paxton, Walter F. ^[1]; McAninch, Patrick T. ^[1]; Achyuthan, Komandoor E. ^[2]; Shin, Sun Hae Ra ^[1]; Monteith, Haley L. ^[1]

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Nano and Microsensors Dept.

Controlling the traffic of molecules and ions across membranes is a critical feature in a number of biologically relevant processes and highly desirable for the development of technologies based on membrane materials. In this study, ion transport behavior of hybrid lipid/polymer membranes was studied in the absence and presence of ion transfer agents. A pH-sensitive fluorophore was used to investigate ion (H⁺/OH^-) permeability across hybrid lipid/polymer membranes as a function of the fraction of amphiphilic block copolymer. It was observed that vesicles with intermediate lipid/polymer ratios tend to be surprisingly more permeable to ion transport than the pure lipid or pure polymer vesicles. Hybrid vesicle permeability could be further modulated with valinomycin, nigericin, or gramicidin A, which significantly expedite the dissipation of externally-imposed pH gradients by facilitating the transport of the rate-limiting co-ions (e.g. K⁺) ions across the membrane. For gramicidin A, ion permeability decreased with increasing polymer mole fraction, and the method of introduction of gramicidin A into the membrane played an important role. Finally, strategies to incorporate biofunctional molecules and facilitate their activity in synthetic systems are highly desirable for developing artificial organelles or other synthetic compartmentalized structures requiring control over molecular traffic across biomimetic membranes.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC)

Grant/Contract Number:: NA0003525

OSTI ID:: 1399496

Alternate ID(s):: OSTI ID: 1549615

Report Number(s):: SAND--2017-2657J; 651645

Journal Information:: Colloids and Surfaces. B, Biointerfaces, Journal Name: Colloids and Surfaces. B, Biointerfaces Vol. 159; ISSN 0927-7765

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Adsorption and fusion of hybrid lipid/polymer vesicles onto 2D and 3D surfaces Paxton, Walter F.; McAninch, Patrick T.; Shin, Sun Hae Ra Soft Matter, Vol. 14, Issue 40 https://doi.org/10.1039/c8sm00343b	journal	January 2018
The combination of block copolymers and phospholipids to form giant hybrid unilamellar vesicles (GHUVs) does not systematically lead to “intermediate” membrane properties Dao, T. P. T.; Fernandes, F.; Fauquignon, M. Soft Matter, Vol. 14, Issue 31 https://doi.org/10.1039/c8sm00547h	journal	January 2018
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Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles

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