Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii

Zurawski, Jeffrey V.; Khatibi, Piyum A.; Akinosho, Hannah O.; Straub, Christopher T.; Compton, Scott H.; Conway, Jonathan M.; Lee, Laura L.; Ragauskas, Arthur J.; Davison, Brian H.; Adams, Michael W. W.; Kelly, Robert M.

doi:10.1128/AEM.00969-17

Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii

Journal Article · Fri Jun 16 00:00:00 EDT 2017 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.00969-17· OSTI ID:1394607

Zurawski, Jeffrey V. ^[1]; Khatibi, Piyum A. ^[1]; Akinosho, Hannah O. ^[2]; Straub, Christopher T. ^[1]; Compton, Scott H. ^[1]; Conway, Jonathan M. ^[1]; Lee, Laura L. ^[1]; ^[3]; ^[4]; Adams, Michael W. W. ^[5]; Kelly, Robert M. ^[1]

North Carolina State Univ., Raleigh, NC (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States); Georgia Inst. of Technology, Atlanta, GA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Georgia, Athens, GA (United States)

ABSTRACT

Improving access to the carbohydrate content of lignocellulose is key to reducing recalcitrance for microbial deconstruction and conversion to fuels and chemicals. Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose. Here, the bioavailability of carbohydrates toC. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(–) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR). Transgenic modification improved carbohydrate solubilization by C. bescii to 15% (2.3-fold) for MYB and to 36% (1.5-fold) for COMT, comparable to the levels achieved for the natural variant, CR (36%). Carbohydrate solubilization was nearly doubled after two consecutive microbial fermentations compared to one microbial step, but it never exceeded 50% overall. Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR]. Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR. Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash). While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1394607

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 17 Vol. 83; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (89)

Enzymatic degradation of lignin-carbohydrate complexes (LCCs): Model studies using a fungal glucuronoyl esterase from Cerrena unicolor: Enzymatic Degradation of Lignin-Carbohydrate Complexes d'Errico, Clotilde; Jørgensen, Jonas O.; Krogh, Kristian B. R. M. Biotechnology and Bioengineering, Vol. 112, Issue 5 https://doi.org/10.1002/bit.25508	journal	January 2015
The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: A modified Simons' staining technique Chandra, Richard; Ewanick, Shannon; Hsieh, Carmen Biotechnology Progress, Vol. 24, Issue 5 https://doi.org/10.1002/btpr.33	journal	September 2008
Optimization of Hydrothermal Pretreatment of Lignocellulosic Biomass in the Bioethanol Production Process Nitsos, Christos K.; Matis, Konstantinos A.; Triantafyllidis, Kostas S. ChemSusChem, Vol. 6, Issue 1 https://doi.org/10.1002/cssc.201200546	journal	November 2012
Genetic Modification of Lignin Biosynthesis for Improved Biofuel Production Hisano, Hiroshi; Nandakumar, Rangaraj; Wang, Zeng-Yu Biofuels https://doi.org/10.1007/978-1-4419-7145-6_12	book	October 2010
Dilute Acid and Autohydrolysis Pretreatment Yang, Bin; Wyman, Charles E.; Mielenz, Jonathan R. Biofuels, Vol. 581, p 103-114 https://doi.org/10.1007/978-1-60761-214-8_8	book	August 2009
Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolv-pretreated lignocellulosic materials Hörmeyer, H. F.; Tailliez, P.; Millet, J. Applied Microbiology and Biotechnology, Vol. 29, Issue 6 https://doi.org/10.1007/BF00260980	journal	December 1988
Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolv-pretreated lignocellulosic materials Hörmeyer, H. F.; Tailliez, P.; Millet, J. Applied Microbiology and Biotechnology, Vol. 29, Issue 6 https://doi.org/10.1007/bf00260980	journal	December 1988
Morphological changes in the cellulose and lignin components of biomass occur at different stages during steam pretreatment Pingali, Sai Venkatesh; O’Neill, Hugh M.; Nishiyama, Yoshiharu Cellulose, Vol. 21, Issue 2 https://doi.org/10.1007/s10570-013-0162-6	journal	January 2014
Genetic modification of lignin biosynthesis for improved biofuel production Hisano, Hiroshi; Nandakumar, Rangaraj; Wang, Zeng-Yu In Vitro Cellular & Developmental Biology - Plant, Vol. 45, Issue 3 https://doi.org/10.1007/s11627-009-9219-5	journal	May 2009
Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB4 for Reduced Biomass Recalcitrance Baxter, Holly L.; Poovaiah, Charleson R.; Yee, Kelsey L. BioEnergy Research, Vol. 8, Issue 3 https://doi.org/10.1007/s12155-014-9570-1	journal	January 2015
Time Course Field Analysis of COMT-Downregulated Switchgrass: Lignification, Recalcitrance, and Rust Susceptibility Baxter, Holly L.; Mazarei, Mitra; Fu, Chunxiang BioEnergy Research, Vol. 9, Issue 4 https://doi.org/10.1007/s12155-016-9751-1	journal	May 2016
Hydrothermal degradation of polymers derived from plants Bobleter, Ortwin Progress in Polymer Science, Vol. 19, Issue 5, p. 797-841 https://doi.org/10.1016/0079-6700(94)90033-7	journal	January 1994
A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol Laser, Mark; Schulman, Deborah; Allen, Stephen G. Bioresource Technology, Vol. 81, Issue 1 https://doi.org/10.1016/S0960-8524(01)00103-1	journal	January 2002
Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition Palmqvist, Eva; Hahn-Hägerdal, Bärbel Bioresource Technology, Vol. 74, Issue 1, p. 25-33 https://doi.org/10.1016/S0960-8524(99)00161-3	journal	August 2000
Biomass yield and quality of 20 switchgrass populations in southern Iowa, USA Lemus, R.; Brummer, E. Charles; Moore, Kenneth J. Biomass and Bioenergy, Vol. 23, Issue 6 https://doi.org/10.1016/S0961-9534(02)00073-9	journal	December 2002
Development of switchgrass (Panicum virgatum) as a bioenergy feedstock in the United States McLaughlin, Samuel B.; Adams Kszos, Lynn Biomass and Bioenergy, Vol. 28, Issue 6 https://doi.org/10.1016/j.biombioe.2004.05.006	journal	June 2005
Features of promising technologies for pretreatment of lignocellulosic biomass Mosier, Nathan; Wyman, Charles; Dale, Bruce Bioresource Technology, Vol. 96, Issue 6, p. 673-686 https://doi.org/10.1016/j.biortech.2004.06.025	journal	April 2005
Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover Ohgren, K.; Bura, R.; Saddler, J. Bioresource Technology, Vol. 98, Issue 13 https://doi.org/10.1016/j.biortech.2006.09.003	journal	September 2007
Pretreatments to enhance the digestibility of lignocellulosic biomass Hendriks, A. T. W. M.; Zeeman, G. Bioresource Technology, Vol. 100, Issue 1 https://doi.org/10.1016/j.biortech.2008.05.027	journal	January 2009
Switchgrass for bioethanol and other value-added applications: A review Keshwani, Deepak R.; Cheng, Jay J. Bioresource Technology, Vol. 100, Issue 4 https://doi.org/10.1016/j.biortech.2008.09.035	journal	February 2009
Comparison of microwaves to fluidized sand baths for heating tubular reactors for hydrothermal and dilute acid batch pretreatment of corn stover Shi, Jian; Pu, Yunqiao; Yang, Bin Bioresource Technology, Vol. 102, Issue 10 https://doi.org/10.1016/j.biortech.2011.03.027	journal	May 2011
Simultaneous saccharification and fermentation of Kanlow switchgrass by thermotolerant Kluyveromyces marxianus IMB3: The effect of enzyme loading, temperature and higher solid loadings Pessani, Naveen K.; Atiyeh, Hasan K.; Wilkins, Mark R. Bioresource Technology, Vol. 102, Issue 22 https://doi.org/10.1016/j.biortech.2011.09.011	journal	November 2011
Degradation of high loads of crystalline cellulose and of unpretreated plant biomass by the thermophilic bacterium Caldicellulosiruptor bescii Basen, Mirko; Rhaesa, Amanda M.; Kataeva, Irina Bioresource Technology, Vol. 152 https://doi.org/10.1016/j.biortech.2013.11.024	journal	January 2014
Plant genetic engineering to improve biomass characteristics for biofuels Sticklen, Mariam Current Opinion in Biotechnology, Vol. 17, Issue 3, p. 315-319 https://doi.org/10.1016/j.copbio.2006.05.003	journal	June 2006
Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels Zeng, Yining; Zhao, Shuai; Yang, Shihui Current Opinion in Biotechnology, Vol. 27 https://doi.org/10.1016/j.copbio.2013.09.008	journal	June 2014
Molecular breeding of switchgrass for use as a biofuel crop Bouton, Joseph H. Current Opinion in Genetics & Development, Vol. 17, Issue 6 https://doi.org/10.1016/j.gde.2007.08.012	journal	December 2007
Engineering of plant cell walls for enhanced biofuel production Loqué, Dominique; Scheller, Henrik V.; Pauly, Markus Current Opinion in Plant Biology, Vol. 25 https://doi.org/10.1016/j.pbi.2015.05.018	journal	June 2015
Plant cell wall reconstruction toward improved lignocellulosic production and processability Abramson, Miron; Shoseyov, Oded; Shani, Ziv Plant Science, Vol. 178, Issue 2, p. 61-72 https://doi.org/10.1016/j.plantsci.2009.11.003	journal	February 2010
Lignocellulosic biomass to bioethanol, a comprehensive review with a focus on pretreatment Haghighi Mood, Sohrab; Hossein Golfeshan, Amir; Tabatabaei, Meisam Renewable and Sustainable Energy Reviews, Vol. 27 https://doi.org/10.1016/j.rser.2013.06.033	journal	November 2013
MYB transcription factors in Arabidopsis Dubos, Christian; Stracke, Ralf; Grotewold, Erich Trends in Plant Science, Vol. 15, Issue 10 https://doi.org/10.1016/j.tplants.2010.06.005	journal	October 2010
Lactate formation in Caldicellulosiruptor saccharolyticus is regulated by the energy carriers pyrophosphate and ATP Willquist, Karin; van Niel, Ed W. J. Metabolic Engineering, Vol. 12, Issue 3 https://doi.org/10.1016/j.ymben.2010.01.001	journal	May 2010
A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol Laser, Mark; Schulman, Deborah; Allen, Stephen G. Bioresource Technology, Vol. 81, Issue 1 https://doi.org/10.1016/s0960-8524(01)00103-1	journal	January 2002
Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition Palmqvist, Eva; Hahn-Hägerdal, Bärbel Bioresource Technology, Vol. 74, Issue 1, p. 25-33 https://doi.org/10.1016/s0960-8524(99)00161-3	journal	August 2000
Biomass yield and quality of 20 switchgrass populations in southern Iowa, USA Lemus, R.; Brummer, E. Charles; Moore, Kenneth J. Biomass and Bioenergy, Vol. 23, Issue 6 https://doi.org/10.1016/s0961-9534(02)00073-9	journal	December 2002
Hydrothermal Pretreatment and Enzymatic Hydrolysis of Prairie Cord Grass Cybulska, Iwona; Lei, Hanwu; Julson, James Energy & Fuels, Vol. 24, Issue 1 https://doi.org/10.1021/ef9009179	journal	January 2010
Hydrothermal Pretreatment of Switchgrass Hu, Zhoujian; Ragauskas, Arthur J. Industrial & Engineering Chemistry Research, Vol. 50, Issue 8 https://doi.org/10.1021/ie101886d	journal	April 2011
Significant Differences in the Hydrolysis Behavior of Amorphous and Crystalline Portions within Microcrystalline Cellulose in Hot-Compressed Water Yu, Yun; Wu, Hongwei Industrial & Engineering Chemistry Research, Vol. 49, Issue 8 https://doi.org/10.1021/ie901925g	journal	April 2010
Lignin modification improves fermentable sugar yields for biofuel production Chen, Fang; Dixon, Richard A. Nature Biotechnology, Vol. 25, Issue 7, p. 759-761 https://doi.org/10.1038/nbt1316	journal	June 2007
Common processes drive the thermochemical pretreatment of lignocellulosic biomass Langan, Paul; Petridis, Loukas; O'Neill, Hugh M. Green Chem., Vol. 16, Issue 1 https://doi.org/10.1039/C3GC41962B	journal	January 2014
Cellulose and lignin colocalization at the plant cell wall surface limits microbial hydrolysis of Populus biomass Dumitrache, Alexandru; Tolbert, Allison; Natzke, Jace Green Chemistry, Vol. 19, Issue 9 https://doi.org/10.1039/C7GC00346C	journal	January 2017
Switchgrass as an energy crop for biofuel production: A review of its ligno-cellulosic chemical properties David, Kasi; Ragauskas, Arthur J. Energy & Environmental Science, Vol. 3, Issue 9 https://doi.org/10.1039/b926617h	journal	January 2010
Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature Kataeva, Irina; Foston, Marcus B.; Yang, Sung-Jae Energy & Environmental Science, Vol. 6, Issue 7 https://doi.org/10.1039/c3ee40932e	journal	January 2013
Common processes drive the thermochemical pretreatment of lignocellulosic biomass Langan, Paul; Petridis, Loukas; O'Neill, Hugh M. Green Chem., Vol. 16, Issue 1 https://doi.org/10.1039/c3gc41962b	journal	January 2014
Cellulose and lignin colocalization at the plant cell wall surface limits microbial hydrolysis of Populus biomass Dumitrache, Alexandru; Tolbert, Allison; Natzke, Jace Green Chemistry, Vol. 19, Issue 9 https://doi.org/10.1039/c7gc00346c	journal	January 2017
Net energy of cellulosic ethanol from switchgrass Schmer, M. R.; Vogel, K. P.; Mitchell, R. B. Proceedings of the National Academy of Sciences, Vol. 105, Issue 2 https://doi.org/10.1073/pnas.0704767105	journal	January 2008
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808 https://doi.org/10.1073/pnas.1100310108	journal	February 2011
Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii Chung, D.; Cha, M.; Guss, A. M. Proceedings of the National Academy of Sciences, Vol. 111, Issue 24, p. 8931-8936 https://doi.org/10.1073/pnas.1402210111	journal	June 2014
Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species Conway, Jonathan M.; Pierce, William S.; Le, Jaycee H. Journal of Biological Chemistry, Vol. 291, Issue 13 https://doi.org/10.1074/jbc.M115.707810	journal	January 2016
Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species Conway, Jonathan M.; Pierce, William S.; Le, Jaycee H. Journal of Biological Chemistry, Vol. 291, Issue 13 https://doi.org/10.1074/jbc.m115.707810	journal	January 2016
The Biology and Agronomy of Switchgrass for Biofuels Parrish, David J.; Fike, John H. Critical Reviews in Plant Sciences, Vol. 24, Issue 5-6 https://doi.org/10.1080/07352680500316433	journal	September 2005
Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis Jin, H. The EMBO Journal, Vol. 19, Issue 22 https://doi.org/10.1093/emboj/19.22.6150	journal	November 2000
Classification of 'Anaerocellum thermophilum' strain DSM 6725 as Caldicellulosiruptor bescii sp. nov. Yang, S. -J.; Kataeva, I.; Wiegel, J. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 9 https://doi.org/10.1099/ijs.0.017731-0	journal	October 2009
Lignin Biosynthesis and Structure Vanholme, R.; Demedts, B.; Morreel, K. Plant Physiology, Vol. 153, Issue 3, p. 895-905 https://doi.org/10.1104/pp.110.155119	journal	May 2010
Monolignol Pathway 4-Coumaric Acid:Coenzyme A Ligases in Populus. trichocarpa : Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes Chen, Hsi-Chuan; Song, Jina; Williams, Cranos M. Plant Physiology, Vol. 161, Issue 3 https://doi.org/10.1104/pp.112.210971	journal	January 2013
Structure-Function Analyses of a Caffeic Acid O -Methyltransferase from Perennial Ryegrass Reveal the Molecular Basis for Substrate Preference Louie, Gordon V.; Bowman, Marianne E.; Tu, Yi The Plant Cell, Vol. 22, Issue 12 https://doi.org/10.1105/tpc.110.077578	journal	December 2010
Thermophilic lignocellulose deconstruction Blumer-Schuette, Sara E.; Brown, Steven D.; Sander, Kyle B. FEMS Microbiology Reviews, Vol. 38, Issue 3 https://doi.org/10.1111/1574-6976.12044	journal	May 2014
C aldicellulosiruptor saccharolyticus transcriptomes reveal consequences of chemical pretreatment and genetic modification of lignocellulose Blumer‐Schuette, Sara E.; Zurawski, Jeffrey V.; Conway, Jonathan M. Microbial Biotechnology, Vol. 10, Issue 6 https://doi.org/10.1111/1751-7915.12494	journal	March 2017
Cell-wall carbohydrates and their modification as a resource for biofuels Pauly, Markus; Keegstra, Kenneth The Plant Journal, Vol. 54, Issue 4, p. 559-568 https://doi.org/10.1111/j.1365-313X.2008.03463.x	journal	May 2008
Cell-wall carbohydrates and their modification as a resource for biofuels Pauly, Markus; Keegstra, Kenneth The Plant Journal, Vol. 54, Issue 4, p. 559-568 https://doi.org/10.1111/j.1365-313x.2008.03463.x	journal	May 2008
Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks Shen, Hui; He, Xianzhi; Poovaiah, Charleson R. New Phytologist, Vol. 193, Issue 1, p. 121-136 https://doi.org/10.1111/j.1469-8137.2011.03922.x	journal	October 2011
Two-year field analysis of reduced recalcitrance transgenic switchgrass Baxter, Holly L.; Mazarei, Mitra; Labbe, Nicole Plant Biotechnology Journal, Vol. 12, Issue 7 https://doi.org/10.1111/pbi.12195	journal	April 2014
Transgenic switchgrass ( Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: a 2-year comparative analysis of field-grown lines modified for target gene or genetic element expression Dumitrache, Alexandru; Natzke, Jace; Rodriguez, Miguel Plant Biotechnology Journal, Vol. 15, Issue 6 https://doi.org/10.1111/pbi.12666	journal	February 2017
The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production Himmel, M. E.; Ding, S.-Y.; Johnson, D. K. Science, Vol. 315, Issue 5813, p. 804-807 https://doi.org/10.1126/science.1137016	journal	February 2007
Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA Brunecky, R.; Alahuhta, M.; Xu, Q. Science, Vol. 342, Issue 6165, p. 1513-1516 https://doi.org/10.1126/science.1244273	journal	December 2013
A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii Lipscomb, Gina L.; Conway, Jonathan M.; Blumer-Schuette, Sara E. Applied and Environmental Microbiology, Vol. 82, Issue 14 https://doi.org/10.1128/AEM.00570-16	journal	May 2016
Phylogenetic, Microbiological, and Glycoside Hydrolase Diversities within the Extremely Thermophilic, Plant Biomass-Degrading Genus Caldicellulosiruptor Blumer-Schuette, S. E.; Lewis, D. L.; Kelly, R. M. Applied and Environmental Microbiology, Vol. 76, Issue 24, p. 8084-8092 https://doi.org/10.1128/AEM.01400-10	journal	October 2010
Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization Zurawski, Jeffrey V.; Conway, Jonathan M.; Lee, Laura L. Applied and Environmental Microbiology, Vol. 81, Issue 20 https://doi.org/10.1128/AEM.01622-15	journal	August 2015
A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii Lipscomb, Gina L.; Conway, Jonathan M.; Blumer-Schuette, Sara E. Applied and Environmental Microbiology, Vol. 82, Issue 14 https://doi.org/10.1128/aem.00570-16	journal	May 2016
Phylogenetic, Microbiological, and Glycoside Hydrolase Diversities within the Extremely Thermophilic, Plant Biomass-Degrading Genus Caldicellulosiruptor Blumer-Schuette, S. E.; Lewis, D. L.; Kelly, R. M. Applied and Environmental Microbiology, Vol. 76, Issue 24, p. 8084-8092 https://doi.org/10.1128/aem.01400-10	journal	October 2010
Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization Zurawski, Jeffrey V.; Conway, Jonathan M.; Lee, Laura L. Applied and Environmental Microbiology, Vol. 81, Issue 20 https://doi.org/10.1128/aem.01622-15	journal	August 2015
Evaluation of ammonia fibre expansion (AFEX) pretreatment for enzymatic hydrolysis of switchgrass harvested in different seasons and locations Bals, Bryan; Rogers, Chad; Jin, Mingjie Biotechnology for Biofuels, Vol. 3, Issue 1 https://doi.org/10.1186/1754-6834-3-1	journal	January 2010
Down-regulation of the caffeic acid O-methyltransferase gene in switchgrass reveals a novel monolignol analog Tschaplinski, Timothy J.; Standaert, Robert F.; Engle, Nancy L. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-71	journal	January 2012
Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach Yee, Kelsey L.; Rodriguez Jr, Miguel; Tschaplinski, Timothy J. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-81	journal	January 2012
Direct glucose production from lignocellulose using Clostridium thermocellum cultures supplemented with a thermostable β-glucosidase Prawitwong, Panida; Waeonukul, Rattiya; Tachaapaikoon, Chakrit Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-184	journal	January 2013
Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-71	journal	January 2013
Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellum strain Yee, Kelsey L.; Rodriguez Jr, Miguel; Thompson, Olivia A. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-75	journal	January 2014
Deletion of Caldicellulosiruptor besciiCelA reveals its crucial role in the deconstruction of lignocellulosic biomass Young, Jenna; Chung, Daehwan; Bomble, Yannick J. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0142-6	journal	October 2014
Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose Chung, Daehwan; Young, Jenna; Cha, Minseok Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0296-x	journal	August 2015
Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment Paye, Julie M. D.; Guseva, Anna; Hammer, Sarah K. Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-015-0412-y	journal	January 2016
Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure Dumitrache, Alexandru; Akinosho, Hannah; Rodriguez, Miguel Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0445-x	journal	February 2016
Natural genetic variability reduces recalcitrance in poplar Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0521-2	journal	May 2016
Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass Li, Mi; Pu, Yunqiao; Yoo, Chang Geun Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-016-0695-7	journal	January 2017
Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of Miscanthus sinensis to Monosaccharides Yoshida, Makoto; Liu, Yuan; Uchida, Satoshi Bioscience, Biotechnology, and Biochemistry, Vol. 72, Issue 3 https://doi.org/10.1271/bbb.70689	journal	March 2008
Hydrothermal Pretreatment and Enzymatic Hydrolysis of Prairie Cord Grass Cybulska, Iwona; Lei, Hanwu; Julson, James L. 2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010 https://doi.org/10.13031/2013.29737	conference	January 2010
The Minor Wall-Networks between Monolignols and Interlinked-Phenolics Predominantly Affect Biomass Enzymatic Digestibility in Miscanthus Li, Zhengru; Zhao, Chunqiao; Zha, Yi PLoS ONE, Vol. 9, Issue 8 https://doi.org/10.1371/journal.pone.0105115	journal	August 2014
Biofuel Component Concentrations and Yields of Switchgrass in South Central U.S. Environments Cassida, K. A.; Muir, J. P.; Hussey, M. A. Crop Science, Vol. 45, Issue 2 https://doi.org/10.2135/cropsci2005.0682	journal	March 2005
Designer Plants for Biofuels: A Review Badhan, Ajay; McAllister, Tim Current Metabolomics, Vol. 4, Issue 1 https://doi.org/10.2174/2213235X03666141226213656	journal	March 2016
Designer Plants for Biofuels: A Review Badhan, Ajay; McAllister, Tim Current Metabolomics, Vol. 4, Issue 1 https://doi.org/10.2174/2213235x03666141226213656	journal	March 2016

Cited By (4)

Lignocellulose solubilization and conversion by extremely thermophilic Caldicellulosiruptor bescii improves by maintaining metabolic activity Straub, Christopher T.; Khatibi, Piyum A.; Otten, Jonathan K. Biotechnology and Bioengineering, Vol. 116, Issue 8 https://doi.org/10.1002/bit.26993	journal	May 2019
Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii Straub, Christopher T.; Khatibi, Piyum A.; Wang, Jack P. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-11376-6	journal	August 2019
Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses Lee, Laura L.; Blumer-Schuette, Sara E.; Izquierdo, Javier A. Applied and Environmental Microbiology, Vol. 84, Issue 9 https://doi.org/10.1128/aem.02694-17	journal	February 2018
Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar Straub, Christopher T.; Bing, Ryan G.; Wang, Jack P. Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-01675-2	journal	March 2020

Similar Records

Lignocellulose solubilization and conversion by extremely thermophilic Caldicellulosiruptor bescii improves by maintaining metabolic activity

Journal Article · Mon May 20 20:00:00 EDT 2019 · Biotechnology and Bioengineering · OSTI ID:1515088

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

Journal Article · Tue Aug 06 20:00:00 EDT 2019 · Nature Communications · OSTI ID:1613208

Related Subjects

60 APPLIED LIFE SCIENCES
Caldicellulosiruptor
extreme thermophiles
lignocellulose
lignocellulose deconstruction and conversion
switchgrass

Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii

Citation Formats

References (89)

Cited By (4)

Similar Records

Related Subjects