Neutron scattering to study membrane systems: from lipid vesicles to living cells.

Conference · Wed Mar 01 00:00:00 EST 2017

OSTI ID:1394599

Nickels, Jonathan D.  ^[1]; Chatterjee, Sneha ^[1]; Stanley, Christopher B.  ^[1]; Qian, Shuo  ^[1]; Cheng, Xiaolin  ^[1]; Myles, Dean A A  ^[1]; Standaert, Robert F.  ^[1]; Elkins, James G.  ^[1]; Katsaras, John  ^[1]

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1. ORNL

The existence and role of lateral lipid organization in biological membranes has been studied and contested for more than 30 years. Lipid domains, or rafts, are hypothesized as scalable compartments in biological membranes, providing appropriate physical environments to their resident membrane proteins. This implies that lateral lipid organization is associated with a range of biological functions, such as protein co-localization, membrane trafficking, and cell signaling, to name just a few. Neutron scattering techniques have proven to be an excellent tool to investigate these structural features in model lipids, and more recently, in living cells. I will discuss our recent work using neutrons to probe the structure and mechanical properties in model lipid systems and our current efforts in using neutrons to probe the structure and organization of the bilayer in a living cell. These efforts in living cells have used genetic and biochemical strategies to generate a large neutron scattering contrast, making the membrane visible. I will present our results showing in vivo bilayer structure and discuss the outlook for this approach.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1394599
• Resource Relation: Conference: Biophysical Society 61st Annual Meeting - New Orleans, Louisiana, United States of America - 2/11/2017 10:00:00 AM-2/15/2017 10:00:00 AM
• Country of Publication: United States
• Language: English