Lateral organization, bilayer asymmetry, and inter-leaflet coupling of biological membranes

Smith, Jeremy C.; Cheng, Xiaolin; Nickels, Jonathan D.

doi:10.1016/j.chemphyslip.2015.07.012

Lateral organization, bilayer asymmetry, and inter-leaflet coupling of biological membranes

Journal Article · Wed Jul 29 00:00:00 EDT 2015 · Chemistry and Physics of Lipids

DOI:https://doi.org/10.1016/j.chemphyslip.2015.07.012· OSTI ID:1256805

Smith, Jeremy C. ^[1]; Cheng, Xiaolin ^[1]; Nickels, Jonathan D. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Understanding of cell membrane organization has evolved significantly from the classic fluid mosaic model. It is now recognized that biological membranes are highly organized structures, with differences in lipid compositions between inner and outer leaflets and in lateral structures within the bilayer plane, known as lipid rafts. These organizing principles are important for protein localization and function as well as cellular signaling. However, the mechanisms and biophysical basis of lipid raft formation, structure, dynamics and function are not clearly understood. One key question, which we focus on in this review, is how lateral organization and leaflet compositional asymmetry are coupled. Detailed information elucidating this question has been sparse because of the small size and transient nature of rafts and the experimental challenges in constructing asymmetric bilayers. Resolving this mystery will require advances in both experimentation and modeling. We discuss here the preparation of model systems along with experimental and computational approaches that have been applied in efforts to address this key question in membrane biology. Furthermore, we seek to place recent and future advances in experimental and computational techniques in context, providing insight into in-plane and transverse organization of biological membranes.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF)

Sponsoring Organization:: DOE Office of Science; USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-00OR22725; FG02-08ER46528

OSTI ID:: 1256805

Alternate ID(s):: OSTI ID: 1247813

Journal Information:: Chemistry and Physics of Lipids, Journal Name: Chemistry and Physics of Lipids Journal Issue: C Vol. 192; ISSN 0009-3084

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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