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Title: c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide

Abstract

We investigated the role of reactive oxygen intermediates (ROIs) and protein kinase C (PKC) in radiation- and H{sub 2}O{sub 2}-evoked c-jun gene expression in human HL-205 cells. This induction of c-jun gene expression could be prevented by pretreatment of the cells with Nacetylcysteine (an antioxidant) or H7 (a PKC and PKA inhibitor) but not by HA1004, a PKA inhibitor, suggesting a role for ROls and PKC in mediating c-jun gene expression. We also investigated potential differences in c-jun gene expression in a panel of normal and tumor cells untreated or treated with ionizing radiation or H{sub 2}O{sub 2}. Treatment with radiation or H{sub 2}O{sub 2} produced a varied response, from some reduction to an increase of more than an order of magnitude in the steady-state level of c-jun mRNA. These data indicate that although induction of c-jun may be a common response to ionizing radiation and H{sub 2}O{sub 2}, this response was reduced or absent in some cell types.

Authors:
; ;
Publication Date:
Research Org.:
Argonne National Lab., IL (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
139193
Report Number(s):
ANL/CMB/CP-80231; CONF-930454-3
ON: DE93017436
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Conference
Resource Relation:
Conference: International seminar on molecular mechanisms in radiation mutagenesis and carcinogenesis, Doorwerth (Netherlands), 19-22 Apr 1993; Other Information: PBD: Jun 1993
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; ONCOGENES; GENE REGULATION; MAN; PEROXIDES; GAMMA RADIATION; COBALT 60; LEUKOCYTES; HYDROGEN PEROXIDE; PHOSPHOTRANSFERASES; ENZYME INHIBITORS; ENZYME INDUCTION; BIOLOGICAL RADIATION EFFECTS

Citation Formats

Collart, F.R., Horio, M., and Huberman, E. c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide. United States: N. p., 1993. Web.
Collart, F.R., Horio, M., & Huberman, E. c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide. United States.
Collart, F.R., Horio, M., and Huberman, E. Tue . "c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide". United States. https://www.osti.gov/servlets/purl/139193.
@article{osti_139193,
title = {c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide},
author = {Collart, F.R. and Horio, M. and Huberman, E.},
abstractNote = {We investigated the role of reactive oxygen intermediates (ROIs) and protein kinase C (PKC) in radiation- and H{sub 2}O{sub 2}-evoked c-jun gene expression in human HL-205 cells. This induction of c-jun gene expression could be prevented by pretreatment of the cells with Nacetylcysteine (an antioxidant) or H7 (a PKC and PKA inhibitor) but not by HA1004, a PKA inhibitor, suggesting a role for ROls and PKC in mediating c-jun gene expression. We also investigated potential differences in c-jun gene expression in a panel of normal and tumor cells untreated or treated with ionizing radiation or H{sub 2}O{sub 2}. Treatment with radiation or H{sub 2}O{sub 2} produced a varied response, from some reduction to an increase of more than an order of magnitude in the steady-state level of c-jun mRNA. These data indicate that although induction of c-jun may be a common response to ionizing radiation and H{sub 2}O{sub 2}, this response was reduced or absent in some cell types.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {6}
}

Conference:
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