Advances in microscale separations towards nanoproteomics applications

Yi, Lian; Piehowski, Paul D.; Shi, Tujin; Smith, Richard D.; Qian, Wei-Jun

doi:10.1016/j.chroma.2017.07.055

Title: Advances in microscale separations towards nanoproteomics applications

Journal Article · Fri Jul 21 00:00:00 EDT 2017 · Journal of Chromatography

DOI:https://doi.org/10.1016/j.chroma.2017.07.055· OSTI ID:1390579

Yi, Lian ^[1]; Piehowski, Paul D. ^[1]; Shi, Tujin ^[1]; Smith, Richard D. ^[1]; Qian, Wei-Jun ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division, Environmental Molecular Sciences Lab.

Microscale separation (e.g., liquid chromatography or capillary electrophoresis) coupled with mass spectrometry (MS) has become the primary tool for advanced proteomics, an indispensable technology for gaining understanding of complex biological processes. In recent decades significant advances have been achieved in MS-based proteomics. But, the current proteomics platforms still face an analytical challenge in overall sensitivity towards nanoproteomics applications for starting materials of less than 1 μg total proteins (e.g., cellular heterogeneity in tissue pathologies). We review recent advances in microscale separation techniques and integrated sample processing strategies that improve the overall sensitivity and proteome coverage of the proteomics workflow, and their contributions towards nanoproteomics applications.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830; AC05-76RL0 1830

OSTI ID:: 1390579

Alternate ID(s):: OSTI ID: 1549572

Report Number(s):: PNNL-SA-125498; PII: S0021967317310567

Journal Information:: Journal of Chromatography, Vol. 1523; ISSN 0021-9673

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

Citation information provided by
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Highly Robust, Automated, and Sensitive Online TiO ₂ -Based Phosphoproteomics Applied To Study Endogenous Phosphorylation in Drosophila melanogaster Pinkse, Martijn W. H.; Mohammed, Shabaz; Gouw, Joost W. Journal of Proteome Research, Vol. 7, Issue 2 https://doi.org/10.1021/pr700605z	journal	February 2008
Making Broad Proteome Protein Measurements in 1−5 min Using High-Speed RPLC Separations and High-Accuracy Mass Measurements Shen, Yufeng; Strittmatter, Eric F.; Zhang, Rui Analytical Chemistry, Vol. 77, Issue 23 https://doi.org/10.1021/ac051257o	journal	December 2005
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NanoLC
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Title: Advances in microscale separations towards nanoproteomics applications

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