The Multi Domain Caldicellulosiruptor bescii CelA Cellulase Excels at the Hydrolysis of Crystalline Cellulose

Brunecky, Roman; Donohoe, Bryon S.; Yarbrough, John M.; Mittal, Ashutosh; Scott, Brian R.; Ding, Hanshu; Taylor II, Larry E.; Russell, Jordan F.; Chung, Daehwan; Westpheling, Janet; Teter, Sarah A.; Himmel, Michael E.; Bomble, Yannick J.

doi:10.1038/s41598-017-08985-w

The Multi Domain Caldicellulosiruptor bescii CelA Cellulase Excels at the Hydrolysis of Crystalline Cellulose

Journal Article · Tue Aug 29 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-08985-w· OSTI ID:1389737

Brunecky, Roman ^[1]; ^[1]; Yarbrough, John M. ^[1]; Mittal, Ashutosh ^[1]; ^[2]; Ding, Hanshu ^[2]; Taylor II, Larry E. ^[1]; Russell, Jordan F. ^[3]; Chung, Daehwan ^[1]; Westpheling, Janet ^[3]; Teter, Sarah A. ^[2]; Himmel, Michael E. ^[1]; Bomble, Yannick J. ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
Novozymes, Inc., Davis, CA (United States)
Univ. of Georgia, Athens, GA (United States)

The crystalline nature of cellulose microfibrils is one of the key factors influencing biomass recalcitrance which is a key technical and economic barrier to overcome to make cellulosic biofuels a commercial reality. To date, all known fungal enzymes tested have great difficulty degrading highly crystalline cellulosic substrates. We have demonstrated that the CelA cellulase from Caldicellulosiruptor bescii degrades highly crystalline cellulose as well as low crystallinity substrates making it the only known cellulase to function well on highly crystalline cellulose. Unlike the secretomes of cellulolytic fungi, which typically comprise multiple, single catalytic domain enzymes for biomass degradation, some bacterial systems employ an alternative strategy that utilizes multi-catalytic domain cellulases. Additionally, CelA is extremely thermostable and highly active at elevated temperatures, unlike commercial fungal cellulases. Furthermore we have determined that the factors negatively affecting digestion of lignocellulosic materials by C. bescii enzyme cocktails containing CelA appear to be significantly different from the performance barriers affecting fungal cellulases. Furthermore, we explore the activity and degradation mechanism of CelA on a variety of pretreated substrates to better understand how the different bulk components of biomass, such as xylan and lignin, impact its performance.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Building Technologies Office (EE-5B)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1389737

Report Number(s):: NREL/JA--2700-68492

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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