Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose

Chung, Daehwan; Young, Jenna; Cha, Minseok; Brunecky, Roman; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

doi:10.1186/s13068-015-0296-x

Title: Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose

Journal Article · Thu Aug 13 00:00:00 EDT 2015 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-015-0296-x· OSTI ID:1225358

Chung, Daehwan ^[1]; Young, Jenna ^[1]; Cha, Minseok ^[1]; Brunecky, Roman ^[2]; Bomble, Yannick J. ^[2]; Himmel, Michael E. ^[2]; Westpheling, Janet ^[1]

Univ. of Georgia, Athens, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

The Caldicellulosiruptor bescii genome encodes a potent set of carbohydrate-active enzymes (CAZymes), found primarily as multi-domain enzymes that exhibit high cellulolytic and hemicellulolytic activity on and allow utilization of a broad range of substrates, including plant biomass without conventional pretreatment. CelA, the most abundant cellulase in the C. bescii secretome, uniquely combines a GH9 endoglucanase and a GH48 exoglucanase in one protein. The most effective commercial enzyme cocktails used in vitro to pretreat biomass are derived from fungal cellulases (cellobiohydrolases, endoglucanases and a β-d-glucosidases) that act synergistically to release sugars for microbial conversion. The C. bescii genome contains six GH5 domains in five different open reading frames. Four exist in multi-domain proteins and two as single catalytic domains. E1 is a GH5 endoglucanase reported to have high specific activity and simple architecture and is active at the growth temperature of C. bescii. E1 is an endo-1,4-β-glucanase linked to a family 2 carbohydrate-binding module shown to bind primarily to cellulosic substrates. As a result, we tested if the addition of this protein to the C. bescii secretome would improve its cellulolytic activity.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1225358

Report Number(s):: NREL/JA-2700-65047

Journal Information:: Biotechnology for Biofuels, Vol. 8, Issue 1; Related Information: Biotechnology for Biofuels; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 24 works

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANAYLYTICAL CHEMISTRY
crystalline cellulose
acidothermus cellulolyticus e1 endoglucanase

Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose [Supplemental Data] Chung, Daehwan; Young, Jenna; Cha, Minseok https://doi.org/10.6084/m9.figshare.c.3646199 The current record is supplemented by this dataset	dataset	August 2015
Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose [Supplementary Data] Chung, Daehwan; Young, Jenna; Cha, Minseok https://doi.org/10.6084/m9.figshare.c.3646199.v1 The current record is supplemented by this collection	collection	August 2015
Additional file 1: of Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose Chung, Daehwan; Young, Jenna; Cha, Minseok https://doi.org/10.6084/m9.figshare.c.3646199_d1 The current record is supplemented by this dataset	dataset	August 2015
Additional file 1: of Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose Chung, Daehwan; Young, Jenna; Cha, Minseok https://doi.org/10.6084/m9.figshare.c.3646199_d1.v1 The current record is supplemented by this dataset	dataset	August 2015