Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses

Joyce, M. Gordon; Wheatley, Adam K.; Thomas, Paul V.; Chuang, Gwo-Yu; Soto, Cinque; Bailer, Robert T.; Druz, Aliaksandr; Georgiev, Ivelin S.; Gillespie, Rebecca A.; Kanekiyo, Masaru; Kong, Wing-Pui; Leung, Kwanyee; Narpala, Sandeep N.; Prabhakaran, Madhu S.; Yang, Eun Sung; Zhang, Baoshan; Zhang, Yi; Asokan, Mangaiarkarasi; Boyington, Jeffrey C.; Bylund, Tatsiana; Darko, Sam; Lees, Christopher R.; Ransier, Amy; Shen, Chen-Hsiang; Wang, Lingshu; Whittle, James R.; Wu, Xueling; Yassine, Hadi M.; Santos, Celia; Matsuoka, Yumiko; Tsybovsky, Yaroslav; Baxa, Ulrich; Mullikin, James C.; Subbarao, Kanta; Douek, Daniel C.; Graham, Barney S.; Koup, Richard A.; Ledgerwood, Julie E.; Roederer, Mario; Shapiro, Lawrence; Kwong, Peter D.; Mascola, John R.; McDermott, Adrian B.

doi:10.1016/j.cell.2016.06.043

Title: Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses

Journal Article · Thu Jul 21 00:00:00 EDT 2016 · Cell

DOI:https://doi.org/10.1016/j.cell.2016.06.043· OSTI ID:1389087

Joyce, M. Gordon; Wheatley, Adam K.; Thomas, Paul V.; Chuang, Gwo-Yu; Soto, Cinque; Bailer, Robert T.; Druz, Aliaksandr; Georgiev, Ivelin S.; Gillespie, Rebecca A.; Kanekiyo, Masaru; Kong, Wing-Pui; Leung, Kwanyee; Narpala, Sandeep N.; Prabhakaran, Madhu S.; Yang, Eun Sung; Zhang, Baoshan; Zhang, Yi; Asokan, Mangaiarkarasi; Boyington, Jeffrey C.; Bylund, Tatsiana more »

Antibodies capable of neutralizing divergent influenza A viruses could form the basis of a universal vaccine. Here, from subjects enrolled in an H5N1 DNA/MIV-prime-boost influenza vaccine trial, we sorted hemagglutinin cross-reactive memory B cells and identified three antibody classes, each capable of neutralizing diverse subtypes of group 1 and group 2 influenza A viruses. Co-crystal structures with hemagglutinin revealed that each class utilized characteristic germline genes and convergent sequence motifs to recognize overlapping epitopes in the hemagglutinin stem. All six analyzed subjects had sequences from at least one multidonor class, and—in half the subjects—multidonor-class sequences were recovered from >40% of cross-reactive B cells. By contrast, these multidonor-class sequences were rare in published antibody datasets. Vaccination with a divergent hemagglutinin can thus increase the frequency of B cells encoding broad influenza A-neutralizing antibodies. We propose the sequence signature-quantified prevalence of these B cells as a metric to guide universal influenza A immunization strategies.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); National Institute of Allergy and Infectious Diseases (NIAID); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Contributing Organization:: NISC Comparative Sequencing Program

Grant/Contract Number:: HHSN261200800001E; W-31-109-Eng-38

OSTI ID:: 1389087

Alternate ID(s):: OSTI ID: 1314264

Journal Information:: Cell, Journal Name: Cell Vol. 166 Journal Issue: 3; ISSN 0092-8674

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 216 works

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