Fully human broadly neutralizing monoclonal antibodies against influenza A viruses generated from the memory B cells of a 2009 pandemic H1N1 influenza vaccine recipient

Journal Article · Sat Jan 19 23:00:00 EST 2013 · Virology

DOI:https://doi.org/10.1016/J.VIROL.2012.09.034· OSTI ID:22150092

Hu, Weibin ^[1]; Chen, Aizhong ^[2]; Miao, Yi ^[3]; Xia, Shengli ^[4]; Ling, Zhiyang; Xu, Ke; Wang, Tongyan ^[1]; Xu, Ying; Cui, Jun; Wu, Hongqiang; Hu, Guiyu; Tian, Lin; Wang, Lingling ^[2]; Shu, Yuelong ^[5]; Ma, Xiaowei ^[6]; Xu, Bianli; Zhang, Jin ^[4]; Lin, Xiaojun ^[6]; Bian, Chao ^[2]; Sun, Bing ^[1]

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1. Molecular Virus Unit, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025 (China)
2. Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China)
3. Shanghai Xuhui Central Hospital, Shanghai 200031 (China)
4. Center for Disease Control and Prevention of Henan Province, Zhengzhou 450016 (China)
5. Chinese Center for Disease Control and Prevention, Beijing 102206 (China)
6. Hualan Biological Bacterin Company, Xinxiang 453003 (China)

Whether the 2009 pandemic H1N1 influenza vaccine can induce heterosubtypic cross-protective anti-hemagglutinin (HA) neutralizing antibodies is an important issue. We obtained a panel of fully human monoclonal antibodies from the memory B cells of a 2009 pandemic H1N1 influenza vaccine recipient. Most of the monoclonal antibodies targeted the HA protein but not the HA1 fragment. Among the analyzed antibodies, seven mAbs exhibited neutralizing activity against several influenza A viruses of different subtypes. The conserved linear epitope targeted by the neutralizing mAbs (FIEGGWTGMVDGWYGYHH) is part of the fusion peptide on HA2. Our work suggests that a heterosubtypic neutralizing antibody response primarily targeting the HA stem region exists in recipients of the 2009 pandemic H1N1 influenza vaccine. The HA stem region contains various conserved neutralizing epitopes with the fusion peptide as an important one. This work may aid in the design of a universal influenza A virus vaccine.

• OSTI ID: 22150092
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 435; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
INFLUENZA
INFLUENZA VIRUSES
MONOCLONAL ANTIBODIES
PEPTIDES
POLYMERASE CHAIN REACTION
RADIATION DOSES
VACCINES