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Title: Multidimensional solid-state NMR spectroscopy of plant cell walls

Abstract

Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely suited for studying native hydrated plant cell walls at the molecular level with chemical resolution. Significant progress has been made in the last five years to elucidate the molecular structures and interactions of cellulose and matrix polysaccharides in plant cell walls. These studies have focused on primary cell walls of growing plants in both the dicotyledonous and grass families, as represented by the model plants Arabidopsis thaliana, Brachypodium distachyon, and Zea mays. To date, these SSNMR results have shown that 1) cellulose, hemicellulose, and pectins form a single network in the primary cell wall; 2) in dicot cell walls, the protein expansin targets the hemicellulose-enriched region of the cellulose microfibril for its wall-loosening function; and 3) primary wall cellulose has polymorphic structures that are distinct from the microbial cellulose structures. This article summarizes these key findings, and points out future directions of investigation to advance our fundamental understanding of plant cell wall structure and function.

Authors:
 [1];  [1];  [1]
  1. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemistry
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1388014
Alternate Identifier(s):
OSTI ID: 1397044
Grant/Contract Number:  
SC0001090
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Solid State Nuclear Magnetic Resonance
Additional Journal Information:
Journal Volume: 78; Journal Issue: C; Related Information: CLSF partners with Pennsylvania State University (lead); North Carolina State University; University of Rhode Island; Virginia Tech University; Journal ID: ISSN 0926-2040
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wang, Tuo, Phyo, Pyae, and Hong, Mei. Multidimensional solid-state NMR spectroscopy of plant cell walls. United States: N. p., 2016. Web. doi:10.1016/j.ssnmr.2016.08.001.
Wang, Tuo, Phyo, Pyae, & Hong, Mei. Multidimensional solid-state NMR spectroscopy of plant cell walls. United States. doi:10.1016/j.ssnmr.2016.08.001.
Wang, Tuo, Phyo, Pyae, and Hong, Mei. Thu . "Multidimensional solid-state NMR spectroscopy of plant cell walls". United States. doi:10.1016/j.ssnmr.2016.08.001. https://www.osti.gov/servlets/purl/1388014.
@article{osti_1388014,
title = {Multidimensional solid-state NMR spectroscopy of plant cell walls},
author = {Wang, Tuo and Phyo, Pyae and Hong, Mei},
abstractNote = {Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely suited for studying native hydrated plant cell walls at the molecular level with chemical resolution. Significant progress has been made in the last five years to elucidate the molecular structures and interactions of cellulose and matrix polysaccharides in plant cell walls. These studies have focused on primary cell walls of growing plants in both the dicotyledonous and grass families, as represented by the model plants Arabidopsis thaliana, Brachypodium distachyon, and Zea mays. To date, these SSNMR results have shown that 1) cellulose, hemicellulose, and pectins form a single network in the primary cell wall; 2) in dicot cell walls, the protein expansin targets the hemicellulose-enriched region of the cellulose microfibril for its wall-loosening function; and 3) primary wall cellulose has polymorphic structures that are distinct from the microbial cellulose structures. This article summarizes these key findings, and points out future directions of investigation to advance our fundamental understanding of plant cell wall structure and function.},
doi = {10.1016/j.ssnmr.2016.08.001},
journal = {Solid State Nuclear Magnetic Resonance},
issn = {0926-2040},
number = C,
volume = 78,
place = {United States},
year = {2016},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 16 works
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