Multidimensional solid-state NMR spectroscopy of plant cell walls

Wang, Tuo; Phyo, Pyae; Hong, Mei

doi:10.1016/j.ssnmr.2016.08.001

Multidimensional solid-state NMR spectroscopy of plant cell walls

Journal Article · Thu Sep 01 00:00:00 EDT 2016 · Solid State Nuclear Magnetic Resonance

DOI:https://doi.org/10.1016/j.ssnmr.2016.08.001· OSTI ID:1388014

Wang, Tuo ^[1]; Phyo, Pyae ^[1]; Hong, Mei ^[1]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemistry

Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely suited for studying native hydrated plant cell walls at the molecular level with chemical resolution. Significant progress has been made in the last five years to elucidate the molecular structures and interactions of cellulose and matrix polysaccharides in plant cell walls. These studies have focused on primary cell walls of growing plants in both the dicotyledonous and grass families, as represented by the model plants Arabidopsis thaliana, Brachypodium distachyon, and Zea mays. To date, these SSNMR results have shown that 1) cellulose, hemicellulose, and pectins form a single network in the primary cell wall; 2) in dicot cell walls, the protein expansin targets the hemicellulose-enriched region of the cellulose microfibril for its wall-loosening function; and 3) primary wall cellulose has polymorphic structures that are distinct from the microbial cellulose structures. This article summarizes these key findings, and points out future directions of investigation to advance our fundamental understanding of plant cell wall structure and function.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0001090

OSTI ID:: 1388014

Alternate ID(s):: OSTI ID: 1397044

Journal Information:: Solid State Nuclear Magnetic Resonance, Journal Name: Solid State Nuclear Magnetic Resonance Journal Issue: C Vol. 78; ISSN 0926-2040

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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