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U.S. Department of Energy
Office of Scientific and Technical Information

Identification of subsurface microorganisms at Yucca Mountain; Fourth quarterly report

Technical Report ·
DOI:https://doi.org/10.2172/138676· OSTI ID:138676
Bacteria isolated from water samples collected in a series of ground water springs have been isolated, enumerated, and identified from twenty six sites. Ten sites were sampled in Death Valley, California and sixteen sites were sampled in Ash Meadows, Nevada. Replicate samples were collected and tested from four locations. All water samples were collected in conjunction with the HRC chemistry group conducting ground water fingerprinting studies. The protocol for collection of samples, as described in the 3rd quarterly report, specified aseptic collection in sterile screw-capped containers and transportation on ice to the HRC microbiology laboratory. All samples were inoculated by spread plating onto R2A (Difco Laboratories, Detroit, MI) bacterial culture medium. the R2A plates were then incubated at 28{degrees} for 5--7 days and colonies wee counted with the aid of a grid template and magnifying lens.
Research Organization:
Nevada Univ., Las Vegas, NV (United States). Harry Reid Center for Environmental Studies
Sponsoring Organization:
USDOE, Washington, DC (United States)
DOE Contract Number:
FC08-90NV10872
OSTI ID:
138676
Report Number(s):
DOE/NV/10872--T69; ON: DE93018969
Country of Publication:
United States
Language:
English