Identification of subsurface microorganisms at Yucca Mountain. Quarterly report, July 1, 1995--September 30, 1995
More than 1100 bacterial isolates were obtained over a two year period from 31 springs in a region along the southern boarder of California and Nevada. Water samples were collected from 17 springs in Ash Meadows National Wildlife Refuge and 14 springs in Death Valley National Park. Bacteria isolated from these samples were subjected to extraction and gas chromatography to determine the cellular fatty acid profile of each isolate. Fatty acid methyl esters (FAME) extracted from cell membranes were separated and classified using the Hewlett Packard by gas chromatography. The FAME profiles of each isolate were then subjected to cluster analysis by the unweighted pair-group method using arithmetic averages. During this quarter the relatedness of FAME patterns of bacterial isolates were examined at the genus level by counting the number of clusters produced in a MIDI dendrogram at a Euclidian distance of 25. This information was then used to determine microbiological relationships among springs.
- Research Organization:
- Nevada Univ., Las Vegas, NV (United States). Harry Reid Center for Environmental Studies
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FC08-90NV10872
- OSTI ID:
- 158476
- Report Number(s):
- DOE/NV/10872--T238; ON: DE96002980; BR: DB0102000/DB0102100
- Country of Publication:
- United States
- Language:
- English
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