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Title: Chromatin organization regulates viral egress dynamics

Abstract

Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.

Authors:
 [1];  [1];  [2];  [2];  [2];  [3];  [4];  [5]; ORCiD logo [6]; ORCiD logo [7];  [8];  [2]
  1. Univ. of Jyvaskyla (Finland). Dept. of Physics
  2. Univ. of Jyvaskyla (Finland). Dept. of Biological and Environmental Science
  3. Univ. of Helsinki (Finland). Inst. of Biotechnology
  4. Univ. of Turku (Finland). Faculty of Medicine
  5. Univ. of Eastern Finland, Kuopio (Finland). Dept. of Applied Physics; Finnish Meteorological Inst. Kuopio (Finland)
  6. Univ. of Jyvaskyla (Finland). Dept. of Physics; ITMO Univ., St. Petersburg (Russia)
  7. Univ. of Jyvaskyla (Finland). Dept. of Physics; Tampere Univ. of Technology (Finland). Dept. of Physics
  8. Univ. of California, San Francisco, CA (United States). Dept. of Anatomy; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Institutes of Health (NIH)
OSTI Identifier:
1379889
Grant/Contract Number:
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; cellular microbiology; herpes virus

Citation Formats

Aho, Vesa, Myllys, Markko, Ruokolainen, Visa, Hakanen, Satu, Mäntylä, Elina, Virtanen, Jori, Hukkanen, Veijo, Kühn, Thomas, Timonen, Jussi, Mattila, Keijo, Larabell, Carolyn A., and Vihinen-Ranta, Maija. Chromatin organization regulates viral egress dynamics. United States: N. p., 2017. Web. doi:10.1038/s41598-017-03630-y.
Aho, Vesa, Myllys, Markko, Ruokolainen, Visa, Hakanen, Satu, Mäntylä, Elina, Virtanen, Jori, Hukkanen, Veijo, Kühn, Thomas, Timonen, Jussi, Mattila, Keijo, Larabell, Carolyn A., & Vihinen-Ranta, Maija. Chromatin organization regulates viral egress dynamics. United States. doi:10.1038/s41598-017-03630-y.
Aho, Vesa, Myllys, Markko, Ruokolainen, Visa, Hakanen, Satu, Mäntylä, Elina, Virtanen, Jori, Hukkanen, Veijo, Kühn, Thomas, Timonen, Jussi, Mattila, Keijo, Larabell, Carolyn A., and Vihinen-Ranta, Maija. 2017. "Chromatin organization regulates viral egress dynamics". United States. doi:10.1038/s41598-017-03630-y. https://www.osti.gov/servlets/purl/1379889.
@article{osti_1379889,
title = {Chromatin organization regulates viral egress dynamics},
author = {Aho, Vesa and Myllys, Markko and Ruokolainen, Visa and Hakanen, Satu and Mäntylä, Elina and Virtanen, Jori and Hukkanen, Veijo and Kühn, Thomas and Timonen, Jussi and Mattila, Keijo and Larabell, Carolyn A. and Vihinen-Ranta, Maija},
abstractNote = {Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.},
doi = {10.1038/s41598-017-03630-y},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = 2017,
month = 6
}

Journal Article:
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