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Title: Chromatin organization regulates viral egress dynamics

Journal Article · · Scientific Reports
 [1];  [1];  [2];  [2];  [2];  [3];  [4];  [5]; ORCiD logo [6]; ORCiD logo [7];  [8];  [2]
  1. Univ. of Jyvaskyla (Finland). Dept. of Physics
  2. Univ. of Jyvaskyla (Finland). Dept. of Biological and Environmental Science
  3. Univ. of Helsinki (Finland). Inst. of Biotechnology
  4. Univ. of Turku (Finland). Faculty of Medicine
  5. Univ. of Eastern Finland, Kuopio (Finland). Dept. of Applied Physics; Finnish Meteorological Inst. Kuopio (Finland)
  6. Univ. of Jyvaskyla (Finland). Dept. of Physics; ITMO Univ., St. Petersburg (Russia)
  7. Univ. of Jyvaskyla (Finland). Dept. of Physics; Tampere Univ. of Technology (Finland). Dept. of Physics
  8. Univ. of California, San Francisco, CA (United States). Dept. of Anatomy; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
+ Show Author Affiliations

Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1379889
Journal Information:
Scientific Reports, Vol. 7, Issue 1; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

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Cited By (4)

Venture from the Interior—Herpesvirus pUL31 Escorts Capsids from Nucleoplasmic Replication Compartments to Sites of Primary Envelopment at the Inner Nuclear Membrane journal November 2017
Imaging cell morphology and physiology using X-rays journal April 2019
The protective function of non-coding DNA in DNA damage accumulation with age and its roles in age-related diseases journal August 2019
Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells journal March 2018

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