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Title: Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development

Abstract

Cell walls of grasses, including cereal crops and biofuel grasses, comprise the majority of plant biomass and intimately influence plant growth, development and physiology. However, the functions of many cell wall synthesis genes, and the relationships among and the functions of cell wall components remain obscure. To better understand the patterns of cell wall accumulation and identify genes that act in grass cell wall biosynthesis, we characterized 30 samples from aerial organs of rice (Oryza sativa cv. Kitaake) at 10 developmental time points, 3-100 d post-germination. Within these samples, we measured 15 cell wall chemical components, enzymatic digestibility and 18 cell wall polysaccharide epitopes/ligands. We also used quantitative reverse transcription-PCR to measure expression of 50 glycosyltransferases, 15 acyltransferases and eight phenylpropanoid genes, many of which had previously been identified as being highly expressed in rice. Most cell wall components vary significantly during development, and correlations among them support current understanding of cell walls. We identified 92 significant correlations between cell wall components and gene expression and establish nine strong hypotheses for genes that synthesize xylans, mixed linkage glucan and pectin components. This work provides an extensive analysis of cell wall composition throughout rice development, identifies genes likely to synthesize grassmore » cell walls, and provides a framework for development of genetically improved grasses for use in lignocellulosic biofuel production and agriculture.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1379223
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Plant and Cell Physiology
Additional Journal Information:
Journal Volume: 57; Journal Issue: 10; Journal ID: ISSN 0032-0781
Publisher:
Japanese Society of Plant Physiologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lin, Fan, Manisseri, Chithra, Fagerström, Alexandra, Peck, Matthew L., Vega-Sánchez, Miguel E., Williams, Brian, Chiniquy, Dawn M., Saha, Prasenjit, Pattathil, Sivakumar, Conlin, Brian, Zhu, Lan, Hahn, Michael G., Willats, William G. T., Scheller, Henrik V., Ronald, Pamela C., and Bartley, Laura E. Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development. United States: N. p., 2016. Web. doi:10.1093/pcp/pcw125.
Lin, Fan, Manisseri, Chithra, Fagerström, Alexandra, Peck, Matthew L., Vega-Sánchez, Miguel E., Williams, Brian, Chiniquy, Dawn M., Saha, Prasenjit, Pattathil, Sivakumar, Conlin, Brian, Zhu, Lan, Hahn, Michael G., Willats, William G. T., Scheller, Henrik V., Ronald, Pamela C., & Bartley, Laura E. Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development. United States. doi:10.1093/pcp/pcw125.
Lin, Fan, Manisseri, Chithra, Fagerström, Alexandra, Peck, Matthew L., Vega-Sánchez, Miguel E., Williams, Brian, Chiniquy, Dawn M., Saha, Prasenjit, Pattathil, Sivakumar, Conlin, Brian, Zhu, Lan, Hahn, Michael G., Willats, William G. T., Scheller, Henrik V., Ronald, Pamela C., and Bartley, Laura E. Mon . "Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development". United States. doi:10.1093/pcp/pcw125.
@article{osti_1379223,
title = {Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development},
author = {Lin, Fan and Manisseri, Chithra and Fagerström, Alexandra and Peck, Matthew L. and Vega-Sánchez, Miguel E. and Williams, Brian and Chiniquy, Dawn M. and Saha, Prasenjit and Pattathil, Sivakumar and Conlin, Brian and Zhu, Lan and Hahn, Michael G. and Willats, William G. T. and Scheller, Henrik V. and Ronald, Pamela C. and Bartley, Laura E.},
abstractNote = {Cell walls of grasses, including cereal crops and biofuel grasses, comprise the majority of plant biomass and intimately influence plant growth, development and physiology. However, the functions of many cell wall synthesis genes, and the relationships among and the functions of cell wall components remain obscure. To better understand the patterns of cell wall accumulation and identify genes that act in grass cell wall biosynthesis, we characterized 30 samples from aerial organs of rice (Oryza sativa cv. Kitaake) at 10 developmental time points, 3-100 d post-germination. Within these samples, we measured 15 cell wall chemical components, enzymatic digestibility and 18 cell wall polysaccharide epitopes/ligands. We also used quantitative reverse transcription-PCR to measure expression of 50 glycosyltransferases, 15 acyltransferases and eight phenylpropanoid genes, many of which had previously been identified as being highly expressed in rice. Most cell wall components vary significantly during development, and correlations among them support current understanding of cell walls. We identified 92 significant correlations between cell wall components and gene expression and establish nine strong hypotheses for genes that synthesize xylans, mixed linkage glucan and pectin components. This work provides an extensive analysis of cell wall composition throughout rice development, identifies genes likely to synthesize grass cell walls, and provides a framework for development of genetically improved grasses for use in lignocellulosic biofuel production and agriculture.},
doi = {10.1093/pcp/pcw125},
journal = {Plant and Cell Physiology},
issn = {0032-0781},
number = 10,
volume = 57,
place = {United States},
year = {2016},
month = {8}
}